has participant NCI-H292 cell NCI-H292 细胞 NICR233 cell NICR233 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000233 Amelogenin：X；CSF1PO：10；D13S317：11，12；D16S539：9，13；D18S51：16；D19S433：12，13.2；D21S11：28；D2S1338：21，24；D3S1358：16；D5S818：13；D7S820：10；D8S1179：11，14；FGA：22，26；TH01：8；TPOX：8，11；vWA：16； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Pulmonary Mucoepidermoid Carcinoma Cells; NCI-H292 NCI-H292 NCI-H292 cell NICR233 P84 Passage at 1: 3 ~ 1: 8; and change fluid once in 2~3 days. RPMI 1640 (w/o Hepes) 10%FBS The cell line is derived from the lymph node metastasis of bronchus mucoepithelioid carcinoma; it is isolated from a defined medium and then cultured in a serum-containing medium. Under the culture conditions, the cells retain the characteristics of mucinous epithelium, which can be indentified by the expression of their ultrastructure and the expression of various differentiation markers of squamous epithelium. This cell supports the growth of HBV and is negative for levodopa decarboxylase detection. This cell can be used as a screening model and human subgenomic fragments can be transfected into cells to study the role of HBV and its own genes in viral hepatitis and hepatocarcinogenesis. The cytokeratin, vimentin and mucicarmine stainings are positive , but the neurofilament triple protein detection is negative. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=233 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:8传代；2~3天换液1次。 Amelogenin：X；CSF1PO：10；D13S317：11，12；D16S539：9，13；D18S51：16；D19S433：12，13.2；D21S11：28；D2S1338：21，24；D3S1358：16；D5S818：13；D7S820：10；D8S1179：11，14；FGA：22，26；TH01：8；TPOX：8，11；vWA：16； A类 NCI-H292 细胞 P84 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人肺粘液上皮样癌细胞；NCI-H292 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞株源自肺支气管黏液上皮样癌的淋巴结转移灶；先用成份限定的培养基分离得到，然后换成含血清的培养基培养。培养条件下该细胞保留了黏液上皮的特性，可从其超微结构的表现和多种鳞状上皮分化标记的表达而判断。该细胞支持HBV的生长，左旋多巴脱羧酶阴性。该细胞可以作为筛选模型，将人类亚基因组片段转染入细胞来研究HBV及其自身基因在病毒性肝炎和肝癌发生中的作用。该细胞角蛋白、波形蛋白、黏液洋红染色阳线，但神经丝三联蛋白阴性。 贴壁生长 vimentin