expresses Hep G2 cell Hep G2 细胞 NICR35 cell NICR35 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000035 44~52，XY Amelogenin：X，Y；CSF1PO：10，11；D13S317：9，13；D16S539：12，13；D18S51：13，14；D19S433：15.2；D21S11：29，31；D2S1338：19，20；D3S1358：15，16；D5S818：11，12；D7S820：10；D8S1179：15，16；FGA：22，25；TH01：9；TPOX：8，9；vWA：17； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Hep G2 Hep G2 cell Hep G2-Species Identification-PUMC Hep G2-cell morphology-PUMC HepG2 HepG2 cell HepG2-Cell Morphology-PUMC Human Hepatocellular Carcinoma Cells; Hep G2 [HepG2] MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA NICR35 PMID: 9169468 Passage at 1: 4 ~ 1: 6; and passage for 2 times a week. The cells are derived from liver cancer tissue of a 15-year-old white teenager. The cells express alpha-fetoprotein, albumin, alpha-2-macroglobulin, alpha-1-antitrypsin, transferrin, alpha-1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen, complement C4, C3 activator, fibrinogen, alpha-1-acid glycoprotein, α-2-HS-glycoprotein, β-lipoprotein, retinol-binding protein, insulin receptor and receptor for insulin-like growth factor; the cells are capable of 3-hydroxy-3-formyl-CoA reductase and hepatic triglyceride lipase activity. The presence of the HBV genome in this cell has not yet been demonstrated. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=35 http://www.cellresource.cn/pdf/20080108100951.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:4~1：6传代；每周2次。 44~52，XY Amelogenin：X，Y；CSF1PO：10，11；D13S317：9，13；D16S539：12，13；D18S51：13，14；D19S433：15.2；D21S11：29，31；D2S1338：19，20；D3S1358：15，16；D5S818：11，12；D7S820：10；D8S1179：15，16；FGA：22，25；TH01：9；TPOX：8，9；vWA：17； A类 C5 Hep G2 细胞 Hep G2-物种鉴定-PUMC Hep G2-细胞形态-PUMC HepG2 细胞 HepG2-细胞形态-PUMC MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS；1%NEAA PMID: 9169468 http://www.cellresource.cn/pdf/20080108100951.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人肝癌细胞；Hep G2 [HepG2] 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞来源于一名15岁的白人少年的肝癌组织。该细胞表达甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗胰蛋白酶、转铁蛋白、α-1-抗凝乳蛋白酶、结合珠蛋白、铜蓝蛋白、纤溶酶原、补体C4、C3激活物、纤维蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、视黄醇结合蛋白；表达胰岛素受体和胰岛素样生长因子IGFⅡ的受体；该细胞具有3-羟基-3-甲酰辅酶A还原酶和肝甘油三酯脂肪酶的活性。目前尚未证明该细胞中有HBV基因组。 贴壁生长 alpha-fetoprotein alpha-2-HS-glycoprotein ceruloplasmin haptoglobin insulin receptor plasminogen alpha-1-antitrypsin alpha-2-macroglobulin