THLE-3 cell THLE-3 细胞 NICR1661 cell NICR1661 细胞 3131C0001000200040 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Clonetics Corporation, Walkersville, MD 21793 (cat # CC3170). The kit contains 500 milliliters of the basic medium and the separately frozen additives. We do not use GA and Epinephrine but add 5 ng/ml EGF and 70 ng/ml Phosphoethanolamine. Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Immortalized Human Liver Cells; THLE-3 NICR1661 Negative PN5 THLE-3 THLE-3 cell The THLE-2 (ATCC CRL-10149) and THLE-3 (ATCC CRL-11233) cell lines were obtained by transfection of normal hepatocytes with SV40 large T antigen. Retroviral vectors containing the Bgl I-Hpa I fragment of SV40 T antigen were introduced into a amphophilic packaging cell line PA317 to produce the virus. THLE-2 and THLE-3 express the characteristic phenotype of normal adult liver epithelial cells. When injected into thymic-free nude mice, they do not form tumors, with a karyotype close to diploid, and do not express alpha-fetoprotein. THLE-2 and THLE-3 metabolize benzo [a] pyrene, N-nitrosodimethylamine, and aflatoxin B1 into the final oncogenic metabolites attached to DNA, thus revealing the role of the cytochrome P450 pathway. THLE also retains other enzymes that are involved in chemical oncolytic metabolism, such as epoxide hydrolase, NADPH cytochrome P450 reductase, superoxide dismutase, catalase, glutathione S-transferases, and glutathione peroxidase. These immortalized human hepatocytes make up the study drug toxicology and Etiological and pathological models of human hepatocellular carcinoma in vitro. Cultures submitted to the ATCC in January 1993 were contaminated Mycoplasma. Their offspring were treated with mycoplasma reagents for 21 days. Six weeks after the treatment, Mycoplasma was tested using Hoechst staining, PCR and standard culture tests. The results were negative. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=1661 A类 Clonetics Corporation, Walkersville, MD 21793的BEGM培养基(目录号CC3170)。 套装包含了500毫升基本培养基及分别冻存的添加剂，我们不用其中的GA和Epinephrine但另外添加了5 ng/ml EGF, 70 ng/ml Phosphoethanolamine。 PN5 THLE-2 (ATCC CRL-10149 和 THLE-3 (ATCC CRL-11233)细胞系是正常肝细胞用SV40大T抗原转染得到。 将含有SV40 T抗原的Bgl I-Hpa I片段的逆转录病毒载体导入兼嗜性包装细胞株PA317中生产病毒。 THLE-2 and THLE-3表达正常成人肝上皮细胞特征性的表型。 当注射到无胸腺裸鼠中时，它们不成瘤，具有接近二倍体的核型，不表达甲胎蛋白。 THLE-2 and THLE-3将benzo[a]pyrene, N-nitrosodimet THLE-3 细胞 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 人肝永生化细胞；THLE-3 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性