U-2 OS cell U-2 OS 细胞 NICR28 cell NICR28 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000028 Amelogenin：X；CSF1PO：13；D13S317：13；D16S539：11，12；D18S51：10.2，12，14；D19S433：15；D21S11：31；D2S1338：20，24；D3S1358：16，18.3；D5S818：11；D7S820：11，12；D8S1179：12，14；FGA：20；TH01：6，9.3；TPOX：11，12；vWA：14，18； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Osteosarcoma Cells; U-2 OS [U2OS; U2-OS; U-2OS] McCoy＇s 5A Media (Modified with Tricine) 10%FBS NICR28 P45 PMID: 9270029 Passage at 1: 3 ~ 1: 6; and passage for 2 to 3 times a week. This cell line (formerly known as 2T) was established and isolated by Ponten J and Saksela E from the moderately differentiated sarcoma of the tibia of a 15-year-old girl in 1964. No virus detected when co-cultured with WI-38 or complement fixation assay (CF method) against monkey vacuolar virus SV40, respiratory syncytial virus RSV or adenovirus. The cells expressed insulin-like growth factor Ⅰ, Ⅱ (IGF-Ⅰ, IGF-Ⅱ) receptor and osteosarcoma-derived growth factor (ODGF). U-2 OS U-2 OS cell U-2OS U-2OS cell U2-OS U2-OS cell U2OS U2OS cell U2OS-Species Identification-PUMC Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=28 http://www.cellresource.cn/pdf/20080108100925.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:6传代；每周2~3次。　 Amelogenin：X；CSF1PO：13；D13S317：13；D16S539：11，12；D18S51：10.2，12，14；D19S433：15；D21S11：31；D2S1338：20，24；D3S1358：16，18.3；D5S818：11；D7S820：11，12；D8S1179：12，14；FGA：20；TH01：6，9.3；TPOX：11，12；vWA：14，18； A类 McCoy＇s 5A Media (Modified with Tricine) 10%FBS P45 PMID: 9270029 U-2 OS 细胞 U-2OS 细胞 U2-OS 细胞 U2OS 细胞 U2OS-物种鉴定-PUMC http://www.cellresource.cn/pdf/20080108100925.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人骨肉瘤细胞；U-2 OS [U2OS；U2-OS；U-2OS] 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞系(原名2T)是由Ponten J和Saksela E在1964年从一名15岁女孩的胫骨中等分化的肉瘤中分离建立的。与WI-38共培养或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的补体结合试验（CF法）未检测到病毒。该细胞表达胰岛素样生长因子Ⅰ、Ⅱ（IGF-Ⅰ、IGF-Ⅱ）受体和骨肉瘤衍生生长因子（ODGF）。 贴壁生长