has participant Kasumi-1 cell Kasumi-1 细胞 NICR1709 cell NICR1709 细胞 3131C0001000700202 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Human Erythroleukemia Cells; Kasumi-1 Kasumi-1 Kasumi-1 cell NICR1709 Negative PN5 Passage at 1: 2. The cells can be overgrown within 3 days. RPMI 1640 (w/o Hepes) qualified fetal bovine serum，20% RPMI 1640 (w/o Hepes) 优质胎牛血清，20% The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This is a leukemia cell line with translocation of chromosome 8: 21. This translocation causes the link of AML1 gene wih the ETO (or MTG8) gene, increasing the expression of the fusion gene AML1-ETO (also known as AML1-MTG or RUNX1-CBF2T1), so the cells produce AML1-ETO protein. This protein downregulates the binding activity of CEBPA mRNA, protein and DNA, which is extremely important for the differentiation of granulocytes. The cell line is established in the peripheral blood of an acute leukemia patient. This cell line is positive for myeloperoxidase staining and shows the morphology of myeloid maturation. Proliferation tests showed that the cultured cells responded to IL-3, IL-6, G-CSF (granulocyte colony stimulating factor), GM-CSF (granulocyte-macrophage colony stimulating factor), but did not respond to IL-1 and IL-5. Dimethylsulfoxide, G-CSF and IL-5 were added to the culture in vitro, and no maturation of granulated or eosinophilic cells was observed. The inducible macrophage like cells can be seen with the addition of phorbol ester. 1,25S- (OH) 2-16 and 23-diene-26-F3-10-nor D3 inhibit its proliferation. http://www.cellresource.cn/fdetail.aspx?id=1709 http://www.cellresource.cn/pdf/20080108101040.pdf myeloblast suspension growth 1:2。3天内可长满。 A类 Kasumi-1 细胞 PN5 RPMI 1640 (w/o Hepes) 优质胎牛血清，20% http://www.cellresource.cn/pdf/20080108101040.pdf 中国科学院上海生命科学研究院细胞资源中心 人红白血病细胞；Kasumi-1 原粒细胞 基础培养基+8%DMSO+20%FBS 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 这是一个带有8：21号染色体转位的白血病细胞株。 这个转位使得AML1基因和ETO (或称 MTG8)基因串联，使融合基因AML1-ETO (也称作 AML1-MTG或 RUNX1-CBF2T1)的表达升高，因而细胞产生嵌合的AML1-ETO蛋白。 这个蛋白下调CEBPA mRNA，蛋白和DNA的结合活性，而这种结合对粒性白细胞的分化是极端重要的。 这株细胞建立于一位急性白血病患者的外周血。 这株细胞髓过氧化物酶阳性，显示其髓性成熟的形态。 增生试验显示，培养的细胞对IL-3、IL-6、G-CSF（粒细胞集落刺激因子）、GM-CSF（粒细胞-巨噬细胞集落刺激因子）有响应，但对IL-1和IL-5没有响应。在体外液体培养中分别加入二甲亚砜、G-CSF、IL-5，也没有观察到粒性或嗜酸性细胞的成熟。 加入佛波酯可以看到诱导出的巨噬细胞样细胞。 1,25S-(OH)2-16,23-diene-26-F3-10-nor D3抑制其增生。 阴性 myeloperoxidase