TF-1 cell TF-1 细胞 NICR118 cell NICR118 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000118 78~104 Amelogenin：X，Y；CSF1PO：12，13；D13S317：8，9；D16S539：9，12；D18S51：13；D19S433：14，16；D21S11：30；D2S1338：19；D3S1358：15；D5S818：13；D7S820：12；D8S1179：11，15；FGA：18，19；TH01：7，9；TPOX：8；vWA：15，17； Base Medium+8%DMSO+20%FBS C6 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Erythroleukemia Cells; TF-1 Maintain the cell concentration between 3 � 104 ~ 5 � 105 cells/ml; and change fluid once in 2~3 days. NICR118 PMID: 9271581 RPMI 1640 (w/o Hepes) 10%FBS；4mM glutamine TF-1 TF-1 cell This cell line was established in 1987 by Kitamura T et al., and was derived from a heparinized bone marrow sample from a 35-year-old Japanese male with severe pancytopenia. Cell growth was entirely dependent on IL-3 or GM-CSF and did not respond to IL-5. Various lymphocytes and cytokines have effects on the cells, such as IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, CSF, LIF, and NGF. The cells did not express glycoprotein A and carbonic anhydrase I. The morphological and cytochemical characteristics of the cell and the constitutive expression of globin gene showed that the cells belonged to erythroid. Hemin and δ aminolevulinic acid induced the synthesis of hemoglobin, and TPA stimulated the cells to differentiate into macrophage-like cells. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=118 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like suspension growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 78~104 Amelogenin：X，Y；CSF1PO：12，13；D13S317：8，9；D16S539：9，12；D18S51：13；D19S433：14，16；D21S11：30；D2S1338：19；D3S1358：15；D5S818：13；D7S820：12；D8S1179：11，15；FGA：18，19；TH01：7，9；TPOX：8；vWA：15，17； A类 C6 PMID: 9271581 RPMI 1640 (w/o Hepes) 10%FBS；4mM glutamine TF-1 细胞 http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人红系白血病细胞；TF-1 培养法（-） 基础培养基+8%DMSO+20%FBS 悬浮生长 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 淋巴母细胞样 维持细胞浓度在3×104~5×105 cells/ml；2~3天换液1次。 该细胞系1987年由Kitamura T等建立，源于一名35岁日本男性的肝素化骨髓抽取样本，该患者具有严重的全血细胞减少症。细胞生长完全依赖于IL-3或GM-CSF，对IL-5无反应。多种淋巴因子和细胞因子对该细胞都有作用，如：IL-1、IL-4、IL-6、IL-9、IL-11、IL-13、CSF、LIF、NGF。该细胞不表达血型糖蛋白A和碳酸酐酶I。由该细胞的形态和细胞化学特征及珠蛋白基因的组成性表达，显示该细胞属于红系。氯高铁血红素和δ氨基乙酰丙酸诱导细胞合成血红蛋白，TPA刺激细胞向巨噬细胞样细胞分