Hep-2 cell Hep-2 细胞 NICR428 cell NICR428 细胞 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation 3111C0002000000056 Amelogenin:X；CSF1PO:9,10；D13S317:12,13.3；D16S539:9,10；D18S51:16；D21S11:27,28；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；PentaD:8,15；PentaE:7,17；TH01:7；TPOX:8,12；vWA:16,18 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Hep-2 Hep-2 cell Hep-2-cell morphology-NIFDC Human Laryngeal Carcinoma Cells; Hep-2 NICR428 National Institute for Food and Drug Control P370 Passage at 1: 3, and passage once in 2-3 days. Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net The cell was initially thought to originate from epithelial carcinoma of the larynx, but subsequent analysis of isozyme analysis, HeLa-labeled chromosomes and DNA fingerprinting found that the cell was contaminated with HeLa; the cells were keratin-positive. adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=428 http://www.cellresource.cn/pdf/20080108100710.pdf 1.Moore AE, et al. Culture characteristics of four permanent lines of human cancer cells. Cancer Res，1955，15: 598-602. 2.Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet, 48: 19-24, 1988. 3.Black FL, et al. Propagation 1:3传代,2-3天传一代 Amelogenin:X；CSF1PO:9,10；D13S317:12,13.3；D16S539:9,10；D18S51:16；D21S11:27,28；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；PentaD:8,15；PentaE:7,17；TH01:7；TPOX:8,12；vWA:16,18 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Hep-2 细胞 Hep-2-细胞形态-NIFDC P370 http://www.cellresource.cn/pdf/20080108100710.pdf 上皮样 中国食品药品检定研究院 人喉癌上皮细胞；Hep-2 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 最初认为这个细胞源自喉上皮癌，但随后通过同工酶分析、HeLa标记染色体和DNA指纹分析发现，该细胞已被HeLa污染；角蛋白阳性。 贴壁生长