THP-1 cell THP-1 细胞 NICR430 cell NICR430 细胞 3111C0002000000058 Amelogenin:X,Y；CSF1PO:11,13；D13S317:13；D16S539:11,12；D18S51:13,14；D21S11:30,31.2；D3S1358:15,17；D5S818:11,12；D7S820:10；D8S1179:10,14；FGA:24,25；PentaD:10,12；PentaE:11,15；TH01:8,9.3；TPOX:8,11；vWA:16 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） Human Acute Monocytic Leukemia Cells; THP-1 Maintain the cell concentration between 2 ~ 4 � 105 ~ 8 � 105/ml, do not exceed 1 � 106/ml; and change fluid once in 2~3 days. NICR430 National Institute for Food and Drug Control RPMI 1640 (w/o Hepes) 10%FBS Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net THP-1 THP-1 cell THP-1-cell morphology-NIFDC The cells were isolated and established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia. The cells can phagocytize latex particles and activated erythrocytes, with no immunoglobulin in both cell membrane and cytoplasm. and express C3R and FcR; The cells can be induced to differentiate into mononuclear cells by phorbol ester TPA and can be used as transfection host. Unknown http://www.cellresource.cn/fdetail.aspx?id=430 http://www.cellresource.cn/pdf/20080108101040.pdf monocyte suspension growth Amelogenin:X,Y；CSF1PO:11,13；D13S317:13；D16S539:11,12；D18S51:13,14；D21S11:30,31.2；D3S1358:15,17；D5S818:11,12；D7S820:10；D8S1179:10,14；FGA:24,25；PentaD:10,12；PentaE:11,15；TH01:8,9.3；TPOX:8,11；vWA:16 A类 RPMI 1640 (w/o Hepes) 10%FBS THP-1 细胞 THP-1-细胞形态-NIFDC http://www.cellresource.cn/pdf/20080108101040.pdf 不详 中国食品药品检定研究院 人急性单核细胞白血病细胞；THP-1 单核细胞 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 悬浮生长 维持细胞浓度在2~4×105~8×105/ml，勿超过1×106/ml；2~3天换液1次。　 该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细胞可以吞噬乳胶颗粒和激活的红细胞，细胞膜和胞浆内均没有免疫球蛋白，表达C3R和FcR；可受佛波酯TPA诱导向单核系方向分化；可作为转染宿主。