HL-60 cell HL-60 细胞 NICR37 cell NICR37 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000037 34~43 Amelogenin：X；CSF1PO：13，14；D13S317：8，11；D16S539：11；D18S51：14，15；D19S433：14；D21S11：29，30；D2S1338：17；D3S1358：16；D5S818：12；D7S820：11，12；D8S1179：13；FGA：22，24；TH01：7，8；TPOX：8，11；vWA：16； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） HL-60 HL-60 cell HL-60-cell morphology-PUMC HL60-Cell Morphology-PUMC Human Acute Promyelocytic Leukemia Cells; HL-60 Maintain the cell concentration between 1 � 105 ~ 1 � 106/ml, and change fluid once every 2~3 day. NICR37 RPMI 1640 (w/o Hepes) 20%FBS The cells were isolated from the peripheral blood of a 36-year-old white woman with acute promyelocytic leukemia by Collins SJ; they can spontaneously differentiate, or differentiate under the stimulation of butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), DMSO(1% to 1.5%), actinomycin D and retinoic acid. TNF-α can be secreted after simulation by PMA. This cell has phagocytosis activity and chemotaxis reaction, oncogene myc-positive, expressing complement receptor and FcR. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=37 http://www.cellresource.cn/pdf/20080108101040.pdf medullary cell-like suspension growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 34~43 Amelogenin：X；CSF1PO：13，14；D13S317：8，11；D16S539：11；D18S51：14，15；D19S433：14；D21S11：29，30；D2S1338：17；D3S1358：16；D5S818：12；D7S820：11，12；D8S1179：13；FGA：22，24；TH01：7，8；TPOX：8，11；vWA：16； A类 C5 HL-60 细胞 HL-60-细胞形态-PUMC HL60-细胞形态-PUMC RPMI 1640 (w/o Hepes) 20%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人急性早幼粒白血病细胞；HL-60 培养法（-） 基础培养基+8%DMSO+20%FBS 悬浮生长 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 维持细胞浓度在1×105~1×106/ml,每2~3天换液1次。 该细胞由Collins SJ从一位患有急性早幼粒细胞性白血病的36岁白人女性的外周血中分离建立；可自发分化，或在丁酸盐、次黄嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1% to 1.5%)、放线菌素D和视黄酸的刺激下发生分化；PMA刺激后可分泌TNF-α。该细胞具有吞噬活性和趋化反应，癌基因myc阳性，表达补体受体和FcR。 髓母细胞样