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    <!-- http://purl.obolibrary.org/obo/CLO_0008827 -->

    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0008827">
        <rdfs:label xml:lang="en">RKO-E6 cell</rdfs:label>
        <rdfs:label xml:lang="zh">RKO-E6 细胞</rdfs:label>
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    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0051949">
        <rdfs:label xml:lang="en">NICR686 cell</rdfs:label>
        <rdfs:label xml:lang="zh">NICR686 细胞</rdfs:label>
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0008827"/>
        <ns2:CLO_0051622 xml:lang="en">3131C0001000700118</ns2:CLO_0051622>
        <ns2:CLO_0051629 xml:lang="en">Base Medium+8%DMSO+20%FBS</ns2:CLO_0051629>
        <ns2:CLO_0051636 xml:lang="en">Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</ns2:CLO_0051636>
        <ns2:CLO_0051633 xml:lang="en">Class A</ns2:CLO_0051633>
        <ns2:CLO_0051637 xml:lang="en">Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn.</ns2:CLO_0051637>
        <ns2:CLO_0051638 xml:lang="en">Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days.</ns2:CLO_0051638>
        <ns2:CLO_0051623 xml:lang="en">Human Colon Cancer Transgenic Cells; RKO-E6</ns2:CLO_0051623>
        <ns2:CLO_0051627 xml:lang="en">MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1</ns2:CLO_0051627>
        <ns2:CLO_0051627 xml:lang="en">MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1</ns2:CLO_0051627>
        <ns2:IAO_0000118 xml:lang="en">NICR686</ns2:IAO_0000118>
        <ns2:CLO_0051630 xml:lang="en">Negative</ns2:CLO_0051630>
        <ns2:CLO_0051628 xml:lang="en">PN5</ns2:CLO_0051628>
        <ns2:IAO_0000118 xml:lang="en">RKO-E6</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">RKO-E6 cell</ns2:IAO_0000118>
        <ns2:CLO_0051626 xml:lang="en">The RKO-E6 cell line is a colon cancer RKO cell line transfected with pCMV-E6 by liposome method. The cells contain a human papillomavirus (HPV) E6 oncogene that is stably integrated and controled by cytomegalovirus promoters. The RKO-E6 cell line, together with its maternal line RKO, can be used to study the p53 deletion-induced changes in cell parameters such as p53-mediated transcription and apoptosis.</ns2:CLO_0051626>
        <rdfs:comment xml:lang="en">The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains).</rdfs:comment>
        <ns2:CLO_0051625 xml:lang="en">adherent growth</ns2:CLO_0051625>
        <ns2:CLO_0051624 xml:lang="en">epithelial cell</ns2:CLO_0051624>
        <rdfs:seeAlso xml:lang="en">http://www.cellresource.cn/fdetail.aspx?id=686</rdfs:seeAlso>
        <ns2:CLO_0051627 xml:lang="en">http://www.cellresource.cn/pdf/20080108100951.pdf</ns2:CLO_0051627>
        <ns2:CLO_0051633 xml:lang="zh">A类</ns2:CLO_0051633>
        <ns2:CLO_0051627 xml:lang="zh">MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1</ns2:CLO_0051627>
        <ns2:CLO_0051628 xml:lang="zh">PN5</ns2:CLO_0051628>
        <ns2:IAO_0000118 xml:lang="zh">RKO-E6 细胞</ns2:IAO_0000118>
        <ns2:CLO_0051626 xml:lang="zh">RKO-E6细胞系是用脂质体法转染pCMV-E6的结肠癌RKO细胞系。 该细胞含有稳定整合受控於巨细胞病毒启动子的人乳头状病毒（HPV）E6癌基因。 RKO-E6细胞系和它的母系RKO一起可用于研究p53缺失引起的细胞参数改变p53介导的转录和凋亡等。</ns2:CLO_0051626>
        <ns2:CLO_0051627 xml:lang="zh">http://www.cellresource.cn/pdf/20080108100951.pdf</ns2:CLO_0051627>
        <ns2:CLO_0051624 xml:lang="zh">上皮细胞</ns2:CLO_0051624>
        <ns2:CLO_0051636 xml:lang="zh">中国科学院上海生命科学研究院细胞资源中心</ns2:CLO_0051636>
        <ns2:CLO_0051623 xml:lang="zh">人结肠癌转基因细胞；RKO-E6</ns2:CLO_0051623>
        <ns2:CLO_0051629 xml:lang="zh">基础培养基+8%DMSO+20%FBS</ns2:CLO_0051629>
        <rdfs:comment xml:lang="zh">本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。</rdfs:comment>
        <ns2:CLO_0051638 xml:lang="zh">消化3-5分钟。1:2。3天内可长满。</ns2:CLO_0051638>
        <ns2:CLO_0051637 xml:lang="zh">联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn</ns2:CLO_0051637>
        <ns2:CLO_0051625 xml:lang="zh">贴壁生长</ns2:CLO_0051625>
        <ns2:CLO_0051630 xml:lang="zh">阴性</ns2:CLO_0051630>
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