T84 cell T84 细胞 NICR1715 cell NICR1715 细胞 3131C0001000700211 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) qualified fetal bovine serum，5% DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，5% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human Colorectal Cancer Lung Metastases Cells; T84 NICR1715 Negative P(53+5) T84 T84 cell T84 cell line is a transplantable human cancer cell line established from the lung metastasis of a 72-year-old male patient with colon cancer. Tumor tissue was inoculated subcutaneously into BALB/c nude mice and transplanted continuously. [26072］During the transplantation of nude mice, the cell lines always maintained the original tissue characteristics of colon cancer. [26072］T84 cell line was established after 23 passages in thymic-free mice. These cell monolayers grew to saturation and exhibited tight junctions and desmosomes between adjacent cells. [1155] There are a lot of reports about polypeptide hormones and neurotransmitters and maintaining directional electrolyte transport. [1155］This cell line presents tight junctions and desmosomes between adjacent cells. [1155］ The cells are keratin-positive in Immunoperoxide staining. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=1715 http://www.cellresource.cn/pdf/20080108100542.pdf A类 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，5% P(53+5) T84 细胞 T84细胞株是从一位72岁男性结肠癌患者的肺转移灶建立的可移植人类癌细胞株。 肿瘤组织皮下接种于BALB/c裸鼠，并连续进行移植。 [26072］ 在裸鼠身上的移植过程中，细胞株始终保持结肠癌的原始组织性状。 [26072］ 在无胸腺小鼠中传代23代后建立了T84细胞株。 这些细胞单层生长到饱和并在接触细胞间展现出紧密连接和桥粒。 [1155] 有很多关于多肽类激素和神经递质并维持定向电解质传输的报道。 [1155］ 这株细胞展现了接触细胞中的紧密连接和桥粒。 [1155］ 角蛋白免疫过氧化物 http://www.cellresource.cn/pdf/20080108100542.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 人结直肠癌肺转移细胞；T84 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性