has_quality has expression HT-29 cell HT-29 细胞 NICR109 cell NICR109 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000109 64~69 Amelogenin：X；CSF1PO：11，12；D13S317：11，12；D16S539：11，12；D18S51：13；D19S433：14；D21S11：29，30；D2S1338：19，23；D3S1358：15，16.3；D5S818：11，12；D7S820：10；D8S1179：10，16；FGA：20，22；TH01：6，9；TPOX：8，9；vWA：17，19； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS HT-29 HT-29 cell HT-29-Cell Morphology-PUMC HT-29-Species Identification-PUMC HT-29-cell morphology-PUMC Human Colorectal Adenocarcinoma Cells; HT-29 NICR109 P143 Passage at 1: 3 ~ 1: 8; and change fluid for 2 to 3 times per week. The cells were isolated from primary tumors in 1964 by Fogh J with transplantation culture and F12 culture medium containing 15% FBS. Recently, the cultured cells of the established strain were cultured in McCoy's 5a medium containing serum. This cell line is tumorigenic in nude mice as well as in steroid-treated hamsters. The cells can synthesize IgA, CEA, TGFβ binding proteins and myxins, expressing urokinase receptor, but no plasminogen activity is detected, and do not express CD4, while galactosylceramide is expressed on the cell surface (a possible replacement receptor for HIV). The oncogene tests for c-myc, K-ras, H-ras, N-ras, Myb, sis, fos are positive; The p53 gene is overexpressed and a G → A mutation occurs at codon 273, resulting in the replacement of Arg → His. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=109 http://www.cellresource.cn/pdf/20080108100542.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1：8传代；每周2~3次换液。 64~69 Amelogenin：X；CSF1PO：11，12；D13S317：11，12；D16S539：11，12；D18S51：13；D19S433：14；D21S11：29，30；D2S1338：19，23；D3S1358：15，16.3；D5S818：11，12；D7S820：10；D8S1179：10，16；FGA：20，22；TH01：6，9；TPOX：8，9；vWA：17，19； A类 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 5%FBS HT-29 细胞 HT-29-物种鉴定-PUMC HT-29-细胞形态-PUMC P143 http://www.cellresource.cn/pdf/20080108100542.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人结直肠腺癌细胞；HT-29 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞是1964年由Fogh J用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的。近来，已建株的培养细胞用含血清的McCoy＇s 5a培养基培养。 该细胞系在裸鼠中成瘤，也能在类固醇处理的地鼠中成瘤。该细胞可合成IgA、CEA、TGFβ结合蛋白和黏液素；表达尿激酶受体，但没有检测到血浆酶原活性；不表达CD4，但细胞表面表达半乳糖神经酰胺（HIV的可能替代受体）。该细胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos阳性；p53基因过表达，并且在273位密码子处发生G→A突变，导致Arg→His替换。 贴壁生长 positive 阳性 FOS MYB NRAS