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    <!-- http://purl.obolibrary.org/obo/CLO_0004283 -->

    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0004283">
        <rdfs:label xml:lang="en">HT-29 cell</rdfs:label>
        <rdfs:label xml:lang="zh">HT-29 细胞</rdfs:label>
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    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0051977">
        <rdfs:label xml:lang="en">NICR1291 cell</rdfs:label>
        <rdfs:label xml:lang="zh">NICR1291 细胞</rdfs:label>
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        <ns3:CLO_0051622 xml:lang="en">3153C0001000000017</ns3:CLO_0051622>
        <ns3:CLO_0051632 xml:lang="en">68-72</ns3:CLO_0051632>
        <ns3:CLO_0037192 xml:lang="en">Amelogenin：X；D8S1179：10, 16；D21S11：29,30；D7S820：10；CSF1PO：11, 12；D3S1358：15, 17；TH01：6, 9；D13S317：11, 12；D16S539：11, 12；D2S1338：19, 23；D19S433：14；vWA：17, 19；THOX：8, 9；D18S51：13；D5S818：11, 12；FGA：20, 22。</ns3:CLO_0037192>
        <ns3:CLO_0051629 xml:lang="en">Base Medium+8%DMSO+20%FBS</ns3:CLO_0051629>
        <ns3:CLO_0051633 xml:lang="en">Class A</ns3:CLO_0051633>
        <ns3:CLO_0051630 xml:lang="en">Fluorescence Method（-）</ns3:CLO_0051630>
        <ns3:IAO_0000118 xml:lang="en">HT-29</ns3:IAO_0000118>
        <ns3:IAO_0000118 xml:lang="en">HT-29 cell</ns3:IAO_0000118>
        <ns3:CLO_0051635 xml:lang="en">HT-29-cell morphology-KCB</ns3:CLO_0051635>
        <ns3:CLO_0051623 xml:lang="en">Human Colorectal Adenocarcinoma Cells; HT-29</ns3:CLO_0051623>
        <ns3:CLO_0051637 xml:lang="en">Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn</ns3:CLO_0051637>
        <ns3:CLO_0051636 xml:lang="en">Kunming Cell Bank, Chinese Academy of Sciences</ns3:CLO_0051636>
        <ns3:IAO_0000118 xml:lang="en">NICR1291</ns3:IAO_0000118>
        <ns3:CLO_0051638 xml:lang="en">Passage at 1: 3; and passage once in 3-4 days</ns3:CLO_0051638>
        <ns3:CLO_0051627 xml:lang="en">RPMI 1640 (w/o Hepes) 15%NBS</ns3:CLO_0051627>
        <rdfs:comment xml:lang="en">The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains).</rdfs:comment>
        <ns3:CLO_0051626 xml:lang="en">The cells were isolated from primary tumors in 1964 by Fogh J with transplantation culture and F12 culture medium containing 15% FBS. Recently, the cultured cells of the established strain were cultured in McCoy&#39;s 5a medium containing serum. This cell line is tumorigenic in nude mice as well as in steroid-treated hamsters. The cells can synthesize IgA, CEA, TGFβ binding proteins and myxins, expressing urokinase receptor, but no plasminogen activity is detected, and do not express CD4, while galactosylceramide is expressed on the cell surface (a possible replacement receptor for HIV). The oncogene tests for c-myc, K-ras, H-ras, N-ras, Myb, sis, fos are positive; The p53 gene is overexpressed and a G → A mutation occurs at codon 273, resulting in the replacement of Arg → His.</ns3:CLO_0051626>
        <ns3:CLO_0051628 xml:lang="en">Unknown</ns3:CLO_0051628>
        <ns3:CLO_0051625 xml:lang="en">adherent growth</ns3:CLO_0051625>
        <ns3:CLO_0051624 xml:lang="en">epithelium-like; polygon</ns3:CLO_0051624>
        <rdfs:seeAlso xml:lang="en">http://www.cellresource.cn/fdetail.aspx?id=1291</rdfs:seeAlso>
        <ns3:CLO_0051627 xml:lang="en">http://www.cellresource.cn/pdf/20080108101040.pdf</ns3:CLO_0051627>
        <ns3:CLO_0051638 xml:lang="zh">1：3传代；3-4天一次</ns3:CLO_0051638>
        <ns3:CLO_0051632 xml:lang="zh">68-72</ns3:CLO_0051632>
        <ns3:CLO_0037192 xml:lang="zh">Amelogenin：X；D8S1179：10, 16；D21S11：29,30；D7S820：10；CSF1PO：11, 12；D3S1358：15, 17；TH01：6, 9；D13S317：11, 12；D16S539：11, 12；D2S1338：19, 23；D19S433：14；vWA：17, 19；THOX：8, 9；D18S51：13；D5S818：11, 12；FGA：20, 22。</ns3:CLO_0037192>
        <ns3:CLO_0051633 xml:lang="zh">A类</ns3:CLO_0051633>
        <ns3:IAO_0000118 xml:lang="zh">HT-29 细胞</ns3:IAO_0000118>
        <ns3:CLO_0051635 xml:lang="zh">HT-29-细胞形态-KCB</ns3:CLO_0051635>
        <ns3:CLO_0051627 xml:lang="zh">RPMI 1640 (w/o Hepes) 15%NBS</ns3:CLO_0051627>
        <ns3:CLO_0051627 xml:lang="zh">http://www.cellresource.cn/pdf/20080108101040.pdf</ns3:CLO_0051627>
        <ns3:CLO_0051624 xml:lang="zh">上皮样；多角</ns3:CLO_0051624>
        <ns3:CLO_0051628 xml:lang="zh">不详</ns3:CLO_0051628>
        <ns3:CLO_0051636 xml:lang="zh">中国科学院昆明细胞库</ns3:CLO_0051636>
        <ns3:CLO_0051623 xml:lang="zh">人结直肠腺癌细胞；HT-29</ns3:CLO_0051623>
        <ns3:CLO_0051629 xml:lang="zh">基础培养基+8%DMSO+20%FBS</ns3:CLO_0051629>
        <rdfs:comment xml:lang="zh">本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。</rdfs:comment>
        <ns3:CLO_0051637 xml:lang="zh">王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn</ns3:CLO_0051637>
        <ns3:CLO_0051630 xml:lang="zh">荧光法（-）</ns3:CLO_0051630>
        <ns3:CLO_0051626 xml:lang="zh">该细胞是1964年由Fogh J用移植培养方法和含15%FBS的F12培养液从原发性肿瘤分离的。近来，已建株的培养细胞用含血清的McCoy＇s 5a培养基培养。 该细胞系在裸鼠中成瘤，也能在类固醇处理的地鼠中成瘤。该细胞可合成IgA、CEA、TGFβ结合蛋白和黏液素；表达尿激酶受体，但没有检测到血浆酶原活性；不表达CD4，但细胞表面表达半乳糖神经酰胺（HIV的可能替代受体）。该细胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos阳性；p53基因过表达，并且在273位密码子处发生G→A突变，导致Arg→His替换。</ns3:CLO_0051626>
        <ns3:CLO_0051625 xml:lang="zh">贴壁生长</ns3:CLO_0051625>
        <foaf:depicted_by rdf:resource="http://www.cellresource.cn/pic/201312269711272.jpg"/>
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    <!-- http://purl.obolibrary.org/obo/CLO_0054406 -->

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        <rdfs:label xml:lang="en">positive</rdfs:label>
        <rdfs:label xml:lang="zh">阳性</rdfs:label>
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    <!-- http://purl.obolibrary.org/obo/OGG_3000002353 -->

    <Class rdf:about="http://purl.obolibrary.org/obo/OGG_3000002353">
        <rdfs:label xml:lang="en">FOS</rdfs:label>
    </Class>
    


    <!-- http://purl.obolibrary.org/obo/OGG_3000004602 -->

    <Class rdf:about="http://purl.obolibrary.org/obo/OGG_3000004602">
        <rdfs:label xml:lang="en">MYB</rdfs:label>
    </Class>
    


    <!-- http://purl.obolibrary.org/obo/OGG_3000004893 -->

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        <rdfs:label xml:lang="en">NRAS</rdfs:label>
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