has_quality produces has expression L Cells cell L Cells 细胞 NICR897 cell NICR897 细胞 3142C0001000000129 Base Medium+8%DMSO+20%FBS C104 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Human Colorectal Adenocarcinoma Cells; LS Isoenzyme Identification Chromosome LS LS cell NICR897 Negative Passage at 1: 3, and passage once in 2-3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell is a trypsin variant of LS 180 colon adenocarcinoma cell line. It is more transmissible than its parents and produces a large amount of carcinoembryonic antigen (CEA) like LS 180. Electron microscopy showed that it had abundant microfilament and cytoplasm mucin vacuoles; serological detection for rectal antigen 3 was positive; p53 antigen expression was negative, but p53 mRNA expression was positive; the cells were cytokeratin-positive; expression for oncogene c-myc, N-myc, H-ras, N-ras, Myb and fos was positive; the expression of oncogene k-ras and sis was not detected; the cells can express IL-10, IL-6 and mucin. adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=897 http://www.cellresource.cn/pdf/20080108100710.pdf 1:3传代,2-3天传一代 A类 C104 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS LS 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 人结直肠腺癌细胞；LS 同工酶鉴定 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 该细胞是LS 180结肠腺癌细胞株的胰蛋白酶化变种。它比亲本更易传代，像LS 180一样生成大量的癌胚抗原(CEA)。电镜研究表明，它有丰富的微丝和胞浆黏液素液泡；血清学检测直肠抗原3阳性；p53抗原表达阴性，但mRNA表达阳性；角蛋白阳性；癌基因c-myc、N-myc、H-ras、N-ras、Myb和fos的表达呈阳性；癌基因k-ras和sis的表达未做检测；可产生IL-10、IL-6和黏蛋白。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性 positive 阳性 FOS MYB NRAS interleukin-6 interleukin-10