immortal human cell line cell 永生的人细胞系细胞 NICR39 cell NICR39 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000039 67~68 Amelogenin：X；CSF1PO：9，10；D13S317：8；D16S539：11，12；D18S51：15，16；D19S433：14，14.2；D21S11：29，30，31；D2S1338：17；D3S1358：16；D5S818：11，12；D7S820：9，11；D8S1179：12；FGA：21，24；TH01：9.3；TPOX：8，9；vWA：16； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Chronic Myeloid Leukemia Cells; K562 K562 K562 cell K562-Species Identification-PUMC K562-cell morphology-PUMC Maintain the cell concentration between 1 � 105 ~ 1 � 106/ml, and change fluid once every 2~3 day. NICR39 RPMI 1640 (w/o Hepes) 10%FBS The cells were isolated by Lozzio from the hydrothorax of a 53-year-old woman with chronic myeloid leukemia in acute phase. The cell was thought to have originated from the granular system and was in a highly undifferentiated stage. Anderson et al considered that it was a erythroleukemia cell line after the study of the cell membrane characteristics. This cell is a highly sensitive in vitro target to natural killer cells and is therefore widely used in this field. The primitive cell of K562 is a malignant tumor cell of hemopoietic system with multidirectional differentiation potential, which can spontaneously differentiate into the identifiable progenitor cells of erythroid, granulocyte and monocyte. The cells express CD7 (25%). Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=39 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like; round suspension growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 67~68 Amelogenin：X；CSF1PO：9，10；D13S317：8；D16S539：11，12；D18S51：15，16；D19S433：14，14.2；D21S11：29，30，31；D2S1338：17；D3S1358：16；D5S818：11，12；D7S820：9，11；D8S1179：12；FGA：21，24；TH01：9.3；TPOX：8，9；vWA：16； A类 C5 K562 细胞 K562-物种鉴定-PUMC K562-细胞形态-PUMC RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人慢性髓系白血病细胞；K562 培养法（-） 基础培养基+8%DMSO+20%FBS 悬浮生长 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 淋巴母细胞样；圆形 维持细胞浓度在1×105~1×106/ml,每2~3天换液1次。 该细胞是由Lozzio从一名53岁的慢性髓细胞性白血病急变期的女性患者的胸水中分离建立的。该细胞曾被认为来源于粒系，处于高度未分化阶段；Anderson等人作了细胞膜特性的研究后，认为该细胞是红白血病细胞系。该细胞是对自然杀伤细胞高度敏感的体外靶标，故而被广泛应用于这方面的研究。K562的原始细胞是一种具有多向分化潜能的造血系统的恶性肿瘤细胞，能自发分化为红系、粒系和单核系的可辨识的祖细胞。该细胞表达CD7（25％）。