immortal human cell line cell 永生的人细胞系细胞 NICR398 cell NICR398 细胞 3111C0002000000026 Amelogenin:X；CSF1PO:9,10；D13S317:8；D16S539:11,12；D18S51:15,16；D21S11:29,31；D3S1358:16；D5S818:11,12；D7S820:9,11；D8S1179:12；FGA:21,24；PentaD:9,13；PentaE:5,14；TH01:9.3；TPOX:8,9；vWA:16 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） Human Chronic Myeloid Leukemia Cells; K562 K 562-cell morphology-NIFDC K562 K562 cell Maintain the cell concentration between 1 � 105 ~ 1 � 106/ml, and change fluid once every 2~3 day. NICR398 National Institute for Food and Drug Control RPMI 1640 (w/o Hepes) new-born calf serum，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net The cells were isolated by Lozzio from the hydrothorax of a 53-year-old woman with chronic myeloid leukemia in acute phase. The cell was thought to have originated from the granular system and was in a highly undifferentiated stage. Anderson et al considered that it was a erythroleukemia cell line after the study of the cell membrane characteristics. This cell is a highly sensitive in vitro target to natural killer cells and is therefore widely used in this field. The primitive cell of K562 is a malignant tumor cell of hemopoietic system with multidirectional differentiation potential, which can spontaneously differentiate into the identifiable progenitor cells of erythroid, granulocyte and monocyte. The cells express CD7 (25%). http://www.cellresource.cn/fdetail.aspx?id=398 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like; round suspension growth Amelogenin:X；CSF1PO:9,10；D13S317:8；D16S539:11,12；D18S51:15,16；D21S11:29,31；D3S1358:16；D5S818:11,12；D7S820:9,11；D8S1179:12；FGA:21,24；PentaD:9,13；PentaE:5,14；TH01:9.3；TPOX:8,9；vWA:16 A类 K 562-细胞形态-NIFDC K562 细胞 RPMI 1640 (w/o Hepes) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 中国食品药品检定研究院 人慢性髓系白血病细胞；K562 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 悬浮生长 淋巴母细胞样；圆形 维持细胞浓度在1×105~1×106/ml,每2~3天换液1次。 该细胞是由Lozzio从一名53岁的慢性髓细胞性白血病急变期的女性患者的胸水中分离建立的。该细胞曾被认为来源于粒系，处于高度未分化阶段；Anderson等人作了细胞膜特性的研究后，认为该细胞是红白血病细胞系。该细胞是对自然杀伤细胞高度敏感的体外靶标，故而被广泛应用于这方面的研究。K562的原始细胞是一种具有多向分化潜能的造血系统的恶性肿瘤细胞，能自发分化为红系、粒系和单核系的可辨识的祖细胞。该细胞表达CD7（25％）。