MRC-5 cell MRC-5 细胞 NICR374 cell NICR374 细胞 3111C0002000000002 Amelogenin:X,Y；CSF1PO:11,12；D13S317:11,14；D16S539:9,11；D18S51:15,21；D21S11:31.2；D3S1358:15,17；D5S818:11,12；D7S820:10,11；D8S1179:13；FGA:21,23；PentaD:12.；PentaE:12,16；TH01:8；TPOX:8；vWA:15 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） Human B,NP,G6PG,MD,AST Human Embryonic Lung Fibroblast; MRC-5 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) new-born calf serum，10%；lactoalbumin hydrolysate，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10%；水解乳蛋白，10% MRC-5 MRC-5 cell MRC-5-cell morphology-NIFDC NICR374 National Institute for Food and Drug Control P33 Passage at 1: 2-1: 4 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net The MRC-5 cell line is derived from normal lung tissue of a 14-week-old male fetus and can be passaged for 42-46 doubling times before aging. adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=374 http://www.cellresource.cn/pdf/20080108100951.pdf 1：2-1：4 Amelogenin:X,Y；CSF1PO:11,12；D13S317:11,14；D16S539:9,11；D18S51:15,21；D21S11:31.2；D3S1358:15,17；D5S818:11,12；D7S820:10,11；D8S1179:13；FGA:21,23；PentaD:12.；PentaE:12,16；TH01:8；TPOX:8；vWA:15 A类 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10%；水解乳蛋白，10% MRC-5 细胞 MRC-5-细胞形态-NIFDC MRC-5细胞系来自14周龄男性胎儿的正常肺组织，该细胞老化前能传代42~46个倍增时间。 P33 http://www.cellresource.cn/pdf/20080108100951.pdf 中国食品药品检定研究院 人B,NP,G6PG,MD,AST 人胚肺成纤维细胞；MRC-5 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 成纤维细胞样 贴壁生长