2V6.11 cell 2V6.11 细胞 NICR483 cell NICR483 细胞 2V6.11 2V6.11 cell 2V6.11-DNA-Fingerprinting-SHCRC 3131C0001000200026 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human Embryonic Kidney Cells; 2V6.11 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 NICR483 Negative PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell line was derived from human embryonic kidney cell line 293 in 2001. 293 cells were transfected with pVgRXR plasmid carrying the Zeocin-resistance marker to obtain 293pVgRXR cells. Subsequently, plasmid pEKORF6 containing the Ad5 E4 ORF6 gene encoding E4 24K was transfected. PEKORF6 is derived from pIndHydro plasmid containing the hygromycin resistance gene. The vector comprises an SV40 viral sequence. The 2V6.11 cell line was initially isolated from the hygromycin-containing culture medium using a clonal ring. Human adenovirus E4 34KDa protein induced by 2V6.11 cells can be detected by immunoblotting. This cell line can be used as a tool for studying adenovirus E4 oncogene adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=483 http://www.cellresource.cn/pdf/20080108100951.pdf 2V6.11 细胞 2V6.11-DNA-指纹-SHCRC A类 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 PN5 http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞样 中国科学院上海生命科学研究院细胞资源中心 人胚肾细胞；2V6.11 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 这株细胞是2001年从人胚肾细胞株293衍化而来。293细胞用携带Zeocin-抗性标记的质粒pVgRXR转染得到293pVgRXR细胞。 随后, 用包含编码E4 24K的Ad5 E4 ORF6基因的质粒pEKORF6转染。pEKORF6从包含潮霉素抗性基因的质粒pIndHydro衍生而来。 载体包含SV40病毒序列。 2V6.11细胞株最初从含潮霉素的培养液中用克隆环分离单克隆得到。 2V6.11细胞诱导表达的人腺病毒E4 34KDa蛋白可通过免疫印迹法检测。 这株细胞可以用作研究腺病毒E4癌基因的工具 阴性