SK-MEL-1 cell SK-MEL-1 细胞 NICR52 cell NICR52 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000052 53~73，XY Amelogenin：X，Y；CSF1PO：12，13；D13S317：11；D16S539：11，12；D18S51：13，16；D19S433：15，16.2；D21S11：29，32.2；D2S1338：19，23；D3S1358：14，16；D5S818：12，13；D7S820：12；D8S1179：16；FGA：18，20；TH01：6；TPOX：11；vWA：16，17； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Skin Melanoma Cells; SK-MEL-1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS Maintain the cell concentration between 1 � 105 ~ 2 � 105, fluid replacement 2 to 3 times a week. NICR52 SK-MEL-1 SK-MEL-1 cell SK-MEL-1-Cell Morphology-PUMC The cells were isolated and established by Oettgen F and his colleagues from the thoracic duct of a 29-year-old white male patient with extensive, fast-advancing malignant melanoma. The cells produced melanin, and the pigment particles in the cells were found to be related to their own synthesis and phagocytosis. Antibodies against this cell line were found in 63% of patients with malignant melanoma and 10% of patients with other diseases. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=52 http://www.cellresource.cn/pdf/20080108100951.pdf spherical suspension growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 53~73，XY Amelogenin：X，Y；CSF1PO：12，13；D13S317：11；D16S539：11，12；D18S51：13，16；D19S433：15，16.2；D21S11：29，32.2；D2S1338：19，23；D3S1358：14，16；D5S818：12，13；D7S820：12；D8S1179：16；FGA：18，20；TH01：6；TPOX：11；vWA：16，17； A类 C5 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS SK-MEL-1 细胞 SK-MEL-1-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108100951.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人皮肤黑色素瘤细胞；SK-MEL-1 培养法（-） 基础培养基+8%DMSO+20%FBS 悬浮生长 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 球形 维持细胞浓度在1×105~2×105,每周补液2~3次。 该细胞由Oettgen F及其同事从一名29岁的患有广泛、快速进展性恶性黑色素瘤的白人男性患者的胸导管中分离建立的。该细胞可产生黑色素，电镜检测发现细胞中色素颗粒与自身合成和吞噬作用相关。在63%的恶性黑色素瘤患者和10%其他疾病患者体内发现了针对该细胞系的抗体。