LNCAP cell LNCAP 细胞 NICR40 cell NICR40 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000040 72~88，XY Amelogenin：X，Y；CSF1PO：10，11；D13S317：10，12；D16S539：11；D18S51：9，11，12；D19S433：13.2，15；D21S11：29，32.2；D2S1338：16，17；D3S1358：16；D5S818：11，12；D7S820：8.1，9.1，10.3；D8S1179：12，14；FGA：19，20；TH01：9；TPOX：8，9；vWA：16，18； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Prostate Cancer Cells; LNCaP LNCaP LNCaP cell LNCaP-Species Identification-PUMC LNCaP-cell morphology-PUMC LNCap-Cell Morphology-PUMC NICR40 P50 Passage at 1: 3 ~ 1: 6; and passage once 5 ~ 7 days. RPMI 1640 (w/o Hepes) 10%FBS The cells were isolated by Horoszewicz JS in 1977 from the living tissue of a 50-year-old white male patient specifically diagnosed with metastatic prostate cancer by fine needle aspiration of the left supraclavicular lymph node. The growth of the cells and the production of acid phosphatase was influenced by 5-α-dihydrotestosterone. The cells do not form uniform monolayers but grow in clusters, so they are repeatedly blown to form individual cells during passage; the cells is not firmly adherent and do not adhere to the confluent state, which would make the culture medium acid quickly; Keep the flask stationary for 48 hours after passage. If the flask is moved, most of the cells would fall off the bottle bottom. If this occurs, it is necessary to reincubate the cells for 24-48 hrs to reattach them to the wall, and then replace the medium after the cells are adherent to the wall. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelium-like; spindle http://www.cellresource.cn/fdetail.aspx?id=40 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:6传代；5~7天1次。 72~88，XY Amelogenin：X，Y；CSF1PO：10，11；D13S317：10，12；D16S539：11；D18S51：9，11，12；D19S433：13.2，15；D21S11：29，32.2；D2S1338：16，17；D3S1358：16；D5S818：11，12；D7S820：8.1，9.1，10.3；D8S1179：12，14；FGA：19，20；TH01：9；TPOX：8，9；vWA：16，18； A类 LNCaP 细胞 LNCaP-物种鉴定-PUMC LNCaP-细胞形态-PUMC LNCap-细胞形态-PUMC P50 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样；梭形 中国医学科学院基础医学研究所基础医学细胞中心 人前列腺癌细胞；LNCaP 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞由Horoszewicz JS于1977年从一名50岁的明确诊断为转移性前列腺癌的白人男性患者的左锁骨上淋巴结细针穿刺的活体组织中分离建立的。5-α-双氢睾酮可影响该细胞的生长和酸性磷酸酶的产生。该细胞不形成均一的单层而是成簇生长，所以传代的时候要反复吹打形成单个细胞；该细胞贴壁不牢，达不到汇合状态，而且会使培养基迅速变酸；传代后48h内一定要保持培养瓶静止.如果此时挪动培养瓶，会使大部分细胞从瓶底脱落。如果出现此种情况，需要重新孵育24~48h使细胞重新贴壁，细胞贴壁后可更换培养基。 贴壁生长