MDA-MB-468 cell MDA-MB-468 细胞 NICR249 cell NICR249 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000249 Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：9；D18S51：17；D19S433：12，13；D21S11：27；D2S1338：17，25；D3S1358：15；D5S818：12；D7S820：8；D8S1179：13；FGA：23；TH01：7；TPOX：8，9；vWA：18； Base Medium+8%DMSO+20%FBS C4 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Breast Cancer Cells; MDA-MB-468 L15: Leibovitz Medium 10%FBS MDA-MB-468 MDA-MB-468 cell MDA-MB-468-cell morphology-PUMC NICR249 Passage at 1: 2 ~ 1: 4; and change fluid for 2~3 times a week. The cells were isolated from the pleural effusion of a 51-year-old black woman with metastatic breast cancer by Cailleau R et al in 1977. Although the G6PD allele of donor tissue was heterozygous, the cell line always showed G6PD A phenotype. The G → A mutation in codon 273 of P53 gene resulted in Arg → His substitution. There were 1×106 EGF receptors on each cell. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=249 http://www.cellresource.cn/pdf/20080108100839.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:2~1:4传代；每周换液2~3次。 Amelogenin：X；CSF1PO：12；D13S317：12；D16S539：9；D18S51：17；D19S433：12，13；D21S11：27；D2S1338：17，25；D3S1358：15；D5S818：12；D7S820：8；D8S1179：13；FGA：23；TH01：7；TPOX：8，9；vWA：18； A类 C4 L15: Leibovitz Medium 10%FBS MDA-MB-468 细胞 MDA-MB-468-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108100839.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人乳腺癌细胞；MDA-MB-468 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞是1977年由Cailleau R等从一位患有转移性乳腺癌的51岁黑人女性的胸腔积液中分离得到的。虽然供体组织的G6PD等位基因杂合，但此细胞株始终表现为G6PD A表型。P53基因273位密码子存在G→A突变，从而导致Arg→His替代。每个细胞上存在1×106个EGF受体。 贴壁生长