IMR-32 cell IMR-32 细胞 NICR711 cell NICR711 细胞 3131C0001000700145 Amelogenin:X,Y；CSF1PO:11,12；D13S317:9；D16S539:8；D18S51:12,15；D19S433:14,15；D21S11:30,31；D2S1338:23,24；D3S1358:16；D5S818:11,12；D7S820:9,10；D8S1179:13；FGA:21,24；TH01:7,9.3；TPOX:11；vWA:15 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3-4 days. Human Neuroblastoma Cells; IMR-32 IMR 32-cell morphology-SHCRC IMR-32 IMR-32 cell IMR-32-DNA-Fingerprinting-SHCRC MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 NICR711 Negative P(52+5) The IMR-32 cells were isolated and established by W.W. Nichols, J. Lee and S. Dwight from a tumor of the lower abdomen of a 13-month-old boy in April 1967. It is diagnosed as neuroblastoma, with organic differentiation in a few areas. The main thing is small neuroblastoma-like cells. The other is large transparent fibroblasts. The cells had been passaged to the 36th passage when they were submitted to ATCC. It has been demonstrated that the cells can be subcultured to over 80 generations. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth http://www.cellresource.cn/fdetail.aspx?id=711 http://www.cellresource.cn/pdf/20080108100951.pdf neuroblastoma, fibroblasts Amelogenin:X,Y；CSF1PO:11,12；D13S317:9；D16S539:8；D18S51:12,15；D19S433:14,15；D21S11:30,31；D2S1338:23,24；D3S1358:16；D5S818:11,12；D7S820:9,10；D8S1179:13；FGA:21,24；TH01:7,9.3；TPOX:11；vWA:15 A类 IMR 32-细胞形态-SHCRC IMR-32 细胞 IMR-32-DNA-指纹-SHCRC IMR-32细胞是1967年四月 W.W. Nichols, J. Lee and S. Dwight从一位13个月大的男婴下腹部肿瘤中分离建株。 诊断认为是神经母细胞瘤，并有少部分区域的器质性分化。 包括两种类型的细胞。 主要的是小的神经母细胞样的细胞。 另一种是大的透明成纤维细胞。 细胞提交到ATCC时已经传到第36代。 已经证明细胞可以传代培养到80代以上。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 P(52+5) http://www.cellresource.cn/pdf/20080108100951.pdf 中国科学院上海生命科学研究院细胞资源中心 人神经母细胞瘤细胞；IMR-32 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3-4天长满。 神经母细胞,成纤维细胞 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性