HK-2 cell HK-2 细胞 NICR159 cell NICR159 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000159 57~60 Amelogenin：X；CSF1PO：13；D13S317：9；D16S539：11，12；D18S51：12；D19S433：15，15.2；D21S11：28，30；D2S1338：17，25；D3S1358：16，17；D5S818：12；D7S820：10，11；D8S1179：10，14；FGA：20，22；TH01：9；TPOX：8，9；vWA：17，18； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS HK-2 HK-2 cell HK-2-Species Identification-PUMC HK-2-cell morphology-PUMC Human Renal Cortex Proximal Convoluted Tubule Epithelial Cells; HK-2 NICR159 P9 Passage at 1: 4; and change fluid once in 2~3 days. The cells, which are proximal convoluted tubules derived from normal kidneys, were immortalized by the introduction of HPV-16 E6/E7 gene. The recombinant retroviral vector pLXSN 16 E6/E7 containing HPV-16 E6/E7 was transfected into the exogenous packaging cells Psi-2, and the virus produced by Psi-2 cells then infected the amphotropic packaging cell line PA317, and finally the virus produced by Psi-2 cells was introduced into normal renal cortical proximal tubule cells. Although neomycin resistance was contained in pLXSN 16 E6/E7, the transduced clones were not screened with G418. Southern and FISH analysis showed that HK-2 cells were derived from the monoclonal. PCR assay confirmed that E6/E7 gene was present in the genome of HK-2 cells. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=159 http://www.cellresource.cn/pdf/20080108100542.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:4传代；2~3天换液1次。 57~60 Amelogenin：X；CSF1PO：13；D13S317：9；D16S539：11，12；D18S51：12；D19S433：15，15.2；D21S11：28，30；D2S1338：17，25；D3S1358：16，17；D5S818：12；D7S820：10，11；D8S1179：10，14；FGA：20，22；TH01：9；TPOX：8，9；vWA：17，18； A类 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 10%FBS HK-2 细胞 HK-2-物种鉴定-PUMC HK-2-细胞形态-PUMC P9 http://www.cellresource.cn/pdf/20080108100542.pdf 上皮细胞样 中国医学科学院基础医学研究所基础医学细胞中心 人肾皮质近曲小管上皮细胞；HK-2 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞属源于正常肾的近曲小管细胞，通过导入HPV-16 E6/E7基因而获得永生化。将含有HPV-16 E6/E7基因的重组的逆转录病毒载体pLXSN 16 E6/E7转染外生包装细胞Psi-2，Psi-2细胞产生的病毒再去感染兼嗜性包装细胞系PA317，最后将PA317产生的病毒颗粒导入正常的肾皮质近曲小管细胞。尽管pLXSN 16 E6/E7中含有新霉素抗性，但未用G418筛选转导克隆。Southern和FISH分析显示HK-2细胞来源于单克隆。PCR检测证实HK-2细胞基因组中含有E6/E7基因。 贴壁生长