HK-2 cell HK-2 细胞 NICR904 cell NICR904 细胞 3142C0001000000136 Base Medium+8%DMSO+20%FBS C5 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS HK-2 HK-2 cell Human Renal Cortex Proximal Convoluted Tubule Epithelial Cells; HK-2 Isoenzyme Identification Chromosome NICR904 Negative Passage at 1: 3, and passage once in 2-3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cells, which are proximal convoluted tubules derived from normal kidneys, were immortalized by the introduction of HPV-16 E6/E7 gene. The recombinant retroviral vector pLXSN 16 E6/E7 containing HPV-16 E6/E7 was transfected into the exogenous packaging cells Psi-2, and the virus produced by Psi-2 cells then infected the amphotropic packaging cell line PA317, and finally the virus produced by Psi-2 cells was introduced into normal renal cortical proximal tubule cells. Although neomycin resistance was contained in pLXSN 16 E6/E7, the transduced clones were not screened with G418. Southern and FISH analysis showed that HK-2 cells were derived from the monoclonal. PCR assay confirmed that E6/E7 gene was present in the genome of HK-2 cells. adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=904 http://www.cellresource.cn/pdf/20080108100710.pdf 1:3传代,2-3天传一代 A类 C5 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS HK-2 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 人肾皮质近曲小管上皮细胞；HK-2 同工酶鉴定 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 该细胞属源于正常肾的近曲小管细胞，通过导入HPV-16 E6/E7基因而获得永生化。将含有HPV-16 E6/E7基因的重组的逆转录病毒载体pLXSN 16 E6/E7转染外生包装细胞Psi-2，Psi-2细胞产生的病毒再去感染兼嗜性包装细胞系PA317，最后将PA317产生的病毒颗粒导入正常的肾皮质近曲小管细胞。尽管pLXSN 16 E6/E7中含有新霉素抗性，但未用G418筛选转导克隆。Southern和FISH分析显示HK-2细胞来源于单克隆。PCR检测证实HK-2细胞基因组中含有E6/E7基因。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性