NCI-H295R cell NCI-H295R 细胞 NICR256 cell NICR256 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000256 Amelogenin：X；CSF1PO：10，12；D13S317：13；D16S539：11；D18S51：17；D19S433：13；D21S11：32.2；D2S1338：25；D3S1358：15，16；D5S818：12；D7S820：9，12；D8S1179：13；FGA：19.2，24；TH01：9.3；TPOX：8；vWA：17，18； Base Medium+8%DMSO+20%FBS C3 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) Nu-Serum I, 2.5%；15 mM HEPES＋0.00625 mg/ml insulin＋0.00625 mg/ml transferrin＋6.25 ng/ml selenium＋1.25 mg/ml bovine serum albumin＋0.00535 mg/ml linoleic acid Human Adrenal Cortical Adenocarcinoma Cells; NCI-H295R NCI-H295R NCI-H295R cell NCI-H295R-Cell Morphology-PUMC NCI-H295R-Species Identification-PUMC NICR256 Passage at 1: 3 ~ 1: 4; and change fluid for 2~3 times a week. This cell line is derived from a multipotent adrenal cancer cell line NCI-H295, which was established by Gazdar AF et al and isolated from adrenal tumors. NCI-H295 cells were cultured under different conditions to obtain NCI-295R cells, and the population doubling time was reduced from 5 days to 2 days. NCI-295 cells were suspended and NCI-H295R was monolayer adherent. NCI-H295R cells retain the ability to produce androgen and respond to angiotensin Ⅱ and potassium ions. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=256 http://www.cellresource.cn/pdf/20080108100542.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:4传代；每周换液2~3次。 Amelogenin：X；CSF1PO：10，12；D13S317：13；D16S539：11；D18S51：17；D19S433：13；D21S11：32.2；D2S1338：25；D3S1358：15，16；D5S818：12；D7S820：9，12；D8S1179：13；FGA：19.2，24；TH01：9.3；TPOX：8；vWA：17，18； A类 C3 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) Nu-Serum I, 2.5%；15 mM HEPES＋0.00625 mg/ml insulin＋0.00625 mg/ml transferrin＋6.25 ng/ml selenium＋1.25 mg/ml bovine serum albumin＋0.00535 mg/ml linoleic acid DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) Nu-Serum I, 2.5%；15 mM HEPES＋0.00625 mg/ml 胰岛素＋0.00625 mg/ml 转铁蛋白＋6.25 ng/ml 硒＋1.25 mg/ml 牛血清白蛋白＋0.00535 mg/ml 亚油酸 NCI-H295R 细胞 NCI-H295R-物种鉴定-PUMC NCI-H295R-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108100542.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人肾上腺皮质腺癌细胞；NCI-H295R 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞系源于多能肾上腺皮质癌细胞系NCI-H295，后者由Gazdar AF等建立，从肾上腺皮质的肿瘤中分离而来。NCI-H295细胞经改变培养条件获得了NCI-295R细胞，群体倍增时间从原来的5天减为2天。NCI-295细胞悬浮生长，而NCI-H295R呈单层贴壁生长。NCI-H295R细胞保留了产生雄激素的能力，对血管紧张素Ⅱ和钾离子有反应。 贴壁生长