769-P cell 769-P 细胞 NICR855 cell NICR855 细胞 3142C0001000000087 769-P 769-P cell Base Medium+8%DMSO+20%FBS C31 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn Human Clear Cell Renal Adenocarcinoma Cells; 769-P Isoenzyme Identification Chromosome MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS NICR855 Negative Passage at 1: 3, and passage once in 2-3 days. The 769-P cell was established in 1975 and derived from a 58-year-old female kidney tissue with primary renal cell adenocarcinoma. The cells are spherical and have a blurry cell edge and a very high nucleoplasmic ratio. The population doubling time is 34.8 hrs after 29 passages, and the cells was able to proliferate on soft agar medium. Immunosuppressed hamster cheek inoculated with the cells produce solid tumors. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=855 http://www.cellresource.cn/pdf/20080108100951.pdf 1:3传代,2-3天传一代 769-P 细胞 769-P细胞建系于1975年，衍生于一个患原发性肾细胞腺癌的58岁女性白人肾组织。该细胞呈球状，并有模糊的细胞边沿和极高的核质比例。细胞连续传29代后群体倍增时间为34.8h，且能在软琼脂培养液上增殖。细胞接种免疫抑制的仓鼠颊产生实体肿瘤。 A类 C31 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 人肾透明细胞腺癌细胞；769-P 同工酶鉴定 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性