Y79 cell Y79 细胞 NICR269 cell NICR269 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000269 40~49 Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Retinoblastoma; Y79 In January 1971, the cells were established by a primary culture of tumors excised from the right eye of a patient by Reid TW and colleagues, in which the patient is of a strong matrilineal inheritance of retinoblastoma. The ultrastructures of the cells, such as nuclear membrane folds, three-layer membrane structure, large coated vesicle, annulate lamellae, microtubules, centrioles, grana, were similar to the original tumors. NICR269 Passage at 1: 2 ~ 1: 4, and change fluid twice a week. RPMI 1640 (w/o Hepes) 20%FBS Y79 Y79 cell Y79-Species Identification-PUMC Y79-cell morphology-PUMC Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=269 http://www.cellresource.cn/pdf/20080108101040.pdf round, growing in clusters suspension growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1971年1月，该细胞由Reid TW及其同事从病人右眼切除的肿瘤进行原代培养建立而成，此病人有很强的视网膜母细胞瘤的母系家族遗传性。该细胞的超微结构，如核膜内折、三层膜结构、大的被膜小泡、环孔板、微管、中心粒、基粒等都与原始肿瘤相似。 1:2~1:4传代，每周2次换液。 40~49 A类 C5 RPMI 1640 (w/o Hepes) 20%FBS Y79 细胞 Y79-物种鉴定-PUMC Y79-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人视网膜母细胞瘤；Y79 圆形，成簇生长 培养法（-） 基础培养基+8%DMSO+20%FBS 悬浮生长 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com