TT cell TT 细胞 NICR268 cell NICR268 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 20~44;65~79 3111C0001CCC000268 Amelogenin：X；CSF1PO：10，13；D13S317：11；D16S539：12，13；D18S51：12；D19S433：14，15；D21S11：29，32.2；D2S1338：17，23；D3S1358：15；D5S818：12，13；D7S820：10，12；D8S1179：15，16；FGA：21，25；TH01：6，9；TPOX：8，11；vWA：16，18； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） F12K 10%FBS Human Medullary Thyroid Carcinoma Cells; TT NICR268 Passage at 1: 3 ~ 1: 4, and change fluid for 2 times a week. TT TT cell TT cells were established by Leong SS et al., from a biopsy sample of a 77 -year-old white woman with medullary thyroid carcinoma. TT cells produce high levels of calcitonin and CEA continuously. The immunoactive calcitonin concentration detected in the medium after medium replacement for 24 hours and 72 hours was 3900 pg/million cells and 7700 pg/million cells, respectively. After 72 hours the CEA accumulation concentration exceeded 27 ng/million cells; The cells were initially cultured in RPMI1640 medium to produce neuropeptides, but it is unknown whether the former effects will occur or not when cultured in F12 medium. TT-cell morphology-PUMC Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=268 http://www.cellresource.cn/pdf/20080108102725.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:4传代，每周换液2次。 20~44;65~79 Amelogenin：X；CSF1PO：10，13；D13S317：11；D16S539：12，13；D18S51：12；D19S433：14，15；D21S11：29，32.2；D2S1338：17，23；D3S1358：15；D5S818：12，13；D7S820：10，12；D8S1179：15，16；FGA：21，25；TH01：6，9；TPOX：8，11；vWA：16，18； A类 C5 F12K 10%FBS TT 细胞 TT-细胞形态-PUMC TT细胞由Leong SS等，自一位77岁白人女性甲状腺髓样癌患者的穿刺活检样本中建立。TT细胞持续产生高水平的降血钙素和CEA。更换培养基后24h和72h，在培养基中检测到的免疫活性的降血钙素浓度分别为3900 pg/106个细胞和7700 pg/106个细胞。 72小时后CEA积累浓度超过27 ng/106个细胞；该细胞最初是在RPMI1640培养液中培养，可产生神经肽，但还不知道在F12培养液中培养是否会产生。 http://www.cellresource.cn/pdf/20080108102725.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人髓样甲状腺肿瘤细胞；TT 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长