TT cell TT 细胞 NICR840 cell NICR840 细胞 3142C0001000000072 Base Medium+8%DMSO+20%FBS C37 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn F12K 10%FBS Human Medullary Thyroid Carcinoma Cells; TT NICR840 Negative Passage at 1: 3, and passage once in 2-3 days. STR Identification TT TT cell TT cells were established by Leong SS et al., from a biopsy sample of a 77 -year-old white woman with medullary thyroid carcinoma. TT cells produce high levels of calcitonin and CEA continuously. The immunoactive calcitonin concentration detected in the medium after medium replacement for 24 hours and 72 hours was 3900 pg/million cells and 7700 pg/million cells, respectively. After 72 hours the CEA accumulation concentration exceeded 27 ng/million cells; The cells were initially cultured in RPMI1640 medium to produce neuropeptides, but it is unknown whether the former effects will occur or not when cultured in F12 medium. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=840 http://www.cellresource.cn/pdf/20080108102725.pdf 1:3传代,2-3天传一代 A类 C37 F12K 10%FBS STR鉴定 TT 细胞 TT细胞由Leong SS等，自一位77岁白人女性甲状腺髓样癌患者的穿刺活检样本中建立。TT细胞持续产生高水平的降血钙素和CEA。更换培养基后24h和72h，在培养基中检测到的免疫活性的降血钙素浓度分别为3900 pg/106个细胞和7700 pg/106个细胞。 72小时后CEA积累浓度超过27 ng/106个细胞；该细胞最初是在RPMI1640培养液中培养，可产生神经肽，但还不知道在F12培养液中培养是否会产生。 http://www.cellresource.cn/pdf/20080108102725.pdf 中国典型培养物保藏中心细胞库 人髓样甲状腺肿瘤细胞；TT 基础培养基+8%DMSO+20%FBS 成纤维细胞样 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性