NCI-H209 cell NCI-H209 细胞 NICR45 cell NICR45 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000045 Amelogenin：X，Y；CSF1PO：11；D13S317：11；D16S539：9，12；D18S51：13；D19S433：14.2，15；D21S11：32.2；D2S1338：17，20；D3S1358：18，20；D5S818：12；D7S820：9；D8S1179：12，13；FGA：20，24；TH01：7，9；TPOX：8；vWA：18，19； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Small Cell Lung Cancer Cells; NCI-H209 NCI-H209 NCI-H209 cell NCI-H209-Cell Morphology-PUMC NCI-H209-cell morphology-PUMC NICR45 Passage at 1: 2; and passage once in 5 to 7 days. RPMI 1640 (w/o Hepes) 20%FBS The cells were isolated from bone marrow metastases of a small cell lung cancer patient by Gazdar AF and his colleagues in 1979, and this bone marrow specimen was obtained before the treatment of the patient. This cell is a typical small cell lung cancer cell, which expresses 4 kinds of biochemical markers of high level: Neurospecific enol, creatine kinase brain band, levodopa decarboxylase and bombesin-like immune activity. The c-myc DNA sequence was not amplified; no large structural DNA abnormalities were found; the cells synthesized p53 mRNA in a comparable amount to normal lung. The cells were suspended and grown in the form of aggregation, and only the cells in aggregation were active, but the cell viability could not be estimated, and there were a lot of cell fragments in the culture medium. The cells express abnormal RB1 protein, and its 706 Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com epithelioid http://www.cellresource.cn/fdetail.aspx?id=45 http://www.cellresource.cn/pdf/20080108101040.pdf suspension aggregation growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:2传代；5~7天1次。 Amelogenin：X，Y；CSF1PO：11；D13S317：11；D16S539：9，12；D18S51：13；D19S433：14.2，15；D21S11：32.2；D2S1338：17，20；D3S1358：18，20；D5S818：12；D7S820：9；D8S1179：12，13；FGA：20，24；TH01：7，9；TPOX：8；vWA：18，19； A类 C5 NCI-H209 细胞 NCI-H209-细胞形态-PUMC RPMI 1640 (w/o Hepes) 20%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人小细胞肺癌细胞；NCI-H209 培养法（-） 基础培养基+8%DMSO+20%FBS 悬浮聚集生长 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞由Gazdar AF及其同事于1979年从一名小细胞肺癌患者的骨髓转移灶中分离建立，该骨髓标本的获取先于患者的治疗。该细胞是一种典型的小细胞性肺癌细胞，表达较高水平的4种生化标志：神经特异性烯醇、肌酸激酶脑型同工酶、左旋多巴脱羧酶、铃蟾肽样免疫活性。c-myc DNA序列没有扩增；未发现大的结构DNA的异常；该细胞合成与正常肺相当量的p53 mRNA。该细胞以聚集体的形式悬浮生长，只有聚集体中的细胞是有活力的，但是细胞活率无法估计，一般培养基中含有大量的细胞碎片。该细胞表达异常的RB1蛋白，其706