expresses NCI-H446 cell NCI-H446 细胞 NICR82 cell NICR82 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000082 Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Small Cell Lung Cancer Cells; NCI-H446 NCI-H446 NCI-H446 cell NCI-H446-Cell Morphology-PUMC NCI-H446-Species Identification-PUMC NCI-H446-cell morphology-PUMC NICR82 Passage at 1: 3 ~ 1: 9; and change fluid for 2~3 times a week. RPMI 1640 (w/o Hepes) 10%FBS The cells were established in 1982 from the pleural effusion of a small cell lung cancer patient by Carney D and Gazdar AF. The original morphology of the cells did not have the characteristics of small cell lung cancer. This cell line was a biochemical and morphological variant of small cell lung cancer, expressing neuron-specific enolase and brain-type creatine kinase isoenzymes and the level of L-dopa decarboxylase, bombyxin, antidiuretic hormone, oxytocin and gastrin-releasing peptide was too low to detect. Compared with normal cells, the c-myc DNA sequence amplication increased about 20 times while that of RNA increased 15 times. The cells was initially subcultured in RPMI 1640 containing 5% FBS with the addition of RPMI 1640, 95% containing 10 nM hydrocortisone, 0.005 mg/ml insulin, 0.01 mg/ml transferrin, 10 nM 17-beta-estradiol, 30 nM sodium selenite. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent/suspension growth, mixed epithelioid http://www.cellresource.cn/fdetail.aspx?id=82 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:9传代；每周换液2~3次。 A类 C5 NCI-H446 细胞 NCI-H446-物种鉴定-PUMC NCI-H446-细胞形态-PUMC RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人小细胞肺癌细胞；NCI-H446 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞是1982年由Carney D和Gazdar AF等从一位小细胞肺癌患者的胸腔积液中建立的。细胞的原始形态并不具有小细胞肺癌特征。这个细胞株是小细胞肺癌的生化和形态学上的变种，表达神经元特有的烯醇酶和脑型肌酸激酶同工酶；左旋多巴脱羧酶、蚕素、抗利尿激素、催产素或胃泌激素释放肽未达到可检测水平。与正常细胞相比，该细胞c-myc DNA序列扩增约20倍，RNA增加15倍。最初传代培养基用含有5%FBS的RPMI1640，另外添加10 nM氢化可的松、0.005 mg/ml胰岛素、0.01 mg/ml转铁蛋白、10 nM 17-beta-雌二醇、30 nM 。 贴壁/悬浮生长，混合 enolase