WISH cell WISH 细胞 NICR470 cell NICR470 细胞 3131C0001000200011 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class B Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human Amniotic Cells; WISH MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 NICR470 Negative PN The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cell was initially thought to originate from normal amniotic membrane, but subsequent analysis of isozyme analysis, HeLa-labeled chromosomes and DNA fingerprinting found that the origin was contaminated with HeLa. The cells are positive for keratin by immunoperoxidase staining. Note: This cell contains a HeLa-labeled chromosome and is constructed from contaminated HeLa cells. WISH WISH cell adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=470 http://www.cellresource.cn/pdf/20080108100951.pdf B类 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 PN WISH 细胞 http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞样 中国科学院上海生命科学研究院细胞资源中心 人羊膜细胞；WISH 基础培养基+8%DMSO+20%FBS 最初以为这株细胞的起源是正常羊膜，但随后通过同工酶分析、HeLA标记染色体和DNA指纹法分析，它的起源是HeLa细胞污染的。 角蛋白免疫过氧化物酶染色阳性。 注意： 这株细胞包含HeLa标记染色体，是从HeLa污染细胞中建株的。 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性