expresses CFPAC-1 cell CFPAC-1 细胞 NICR245 cell NICR245 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000245 54~71 Amelogenin：X，Y；CSF1PO：10；D13S317：12；D16S539：9，11；D18S51：12；D19S433：13，15；D21S11：30，31.2；D2S1338：18，23；D3S1358：16；D5S818：10，11；D7S820：8，10；D8S1179：11，15；FGA：21，22；TH01：8；TPOX：8；vWA：17； Base Medium+8%DMSO+20%FBS C4 CFPAC-1 CFPAC-1 cell CFPAC-1-Species Identification-PUMC CFPAC-1-cell morphology-PUMC Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Pancreatic Ductal Carcinoma Cells; CFPAC-1 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS；1.5g/L NaHCO3 NICR245 Passage at 1: 3 ~ 10; and change fluid once in 2~3 days. The cells are derived from the liver metastasis of 26-year-old white male with cystic fibrosis (CF), expressing cystic fibrosis transmembrane conductance regulator (CFTR), with microvillous polarization at the cell tip, and express of cytokeratin and carcinoembryonic antigen with characteristic of pancreatic duct cells; the doubling time is 30~32hrs. This cell is a subtriploid cell line and tumorigenic in thymic mice when passaged to the 24 generation. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=245 http://www.cellresource.cn/pdf/20080108100822.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~10传代；2~3天换液1次。 54~71 Amelogenin：X，Y；CSF1PO：10；D13S317：12；D16S539：9，11；D18S51：12；D19S433：13，15；D21S11：30，31.2；D2S1338：18，23；D3S1358：16；D5S818：10，11；D7S820：8，10；D8S1179：11，15；FGA：21，22；TH01：8；TPOX：8；vWA：17； A类 C4 CFPAC-1 细胞 CFPAC-1-物种鉴定-PUMC CFPAC-1-细胞形态-PUMC IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS；1.5g/L NaHCO3 http://www.cellresource.cn/pdf/20080108100822.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人胰腺导管癌细胞；CFPAC-1 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞建自26岁白人男性囊性纤维变性（CF）的肝转移灶；表达囊性纤维变跨膜传导调节因子（CFTR）；细胞顶端微绒毛极化；表达胰腺管细胞特征性的细胞角蛋白和癌胚抗原；倍增时间为30~32小时。该细胞是亚3倍体的细胞系；传代至24代时在无胸腺小鼠中致瘤。 贴壁生长 cystic fibrosis transmembrane conductance regulator (human)