HBL-100 cell HBL-100 细胞 NICR469 cell NICR469 细胞 3131C0001000200010 Amelogenin:X；CSF1PO:10；D13S317:12；D16S539:9,12；D18S51:16；D19S433:15；D21S11:28,30；D2S1338:18,24；D3S1358:14,16；D5S818:11,12；D7S820:8,12；D8S1179:12,15；FGA:25；TH01:6,8；TPOX:8；vWA:16 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. HBL-100 HBL-100 cell HBL-100 is an epithelial cell line, E.V. Gaffney and his colleagues were created from the milk of a donor without a family history of breast cancer. The karyotype of the cultured cells was abnormal at the 7th generation. The microfilaments, tension fibrils and desmosomes were observed by electron microscope. Southern blot showed that there was an integrated SV40 gene in the cells, which could not be considered as normal cells. HBL-100-cell morphology-SHCRC HBL100 HBL100 cell Human Mammary Epithelial Cells Integrated with SV40 Gene; HBL-100 [HBL100] NICR469 Negative PN RPMI 1640 (w/o Hepes) fetal bovine serum，10% RPMI 1640 (w/o Hepes) 胎牛血清，10% The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=469 http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:10；D13S317:12；D16S539:9,12；D18S51:16；D19S433:15；D21S11:28,30；D2S1338:18,24；D3S1358:14,16；D5S818:11,12；D7S820:8,12；D8S1179:12,15；FGA:25；TH01:6,8；TPOX:8；vWA:16 A类 HBL-100 细胞 HBL-100-细胞形态-SHCRC HBL-100是一株上皮细胞，E.V. Gaffney及其同事从一位没有乳癌家族史的供者乳汁中建立。 培养出来的细胞染色体组型在第7代时就不正常。电镜照片显示有微丝、张力原纤维和桥粒。 Southern转移表明有整合型SV40病毒基因，不可以当作正常细胞。 HBL100 细胞 PN RPMI 1640 (w/o Hepes) 胎牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 上皮细胞样 中国科学院上海生命科学研究院细胞资源中心 人整合SV40基因的乳腺上皮细胞；HBL-100 [HBL100] 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性