RWPE-1 cell RWPE-1 细胞 NICR1659 cell NICR1659 细胞 3131C0001000200037 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human Normal Prostate Epithelial Cells; RWPE-1 NICR1659 Negative P(52+5) RWPE-1 RWPE-1 cell The basic medium for this strain of the cells is part of a kit provided by Invitrogen (GIBCO): keratinocyte serum-free medium K-SFM, kit Cat # 17005-042. The two additives (bovine pituitary extract BPE and human recombinant epidermal growth factor EGF) required for the growth of this strain were supplied with the kit. To prepare complete growth medium, the following ingredients are needed to add: 0.05 mg/ml BPE - 5 ng/ml EGF with K-SFM - supplied with K-SFM. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). Tumor suppressor gene: P53+ [PubMed: 9214605] pRB+ [PubMed: 9214605] The epithelial cells in the surrounding area of a normal male prostate tissue section were transformed with a single copy of human papillomavirus 18 (HPV-18) to establish RWPE-1 (ATCC PublRL-11609) cell line[PubMed: 9214605]. In three-dimensional matrigel culture, RWPE-1 cells form adenocytes and secrete PSA into the medium under the effect of androgen. [PubMed: 11170142]. When male nude mice are injected in admixture with Matrigel or stromal cells, RWPE-1 cells can also form adenosine.[PubMed: 11304724]and produce PSA. Ki-ras gene in cells derived from RWPE-1 were tranfected again with the Kirstin mouse tumoe virus, then esablishing the tumorigenic RWPE-2 cell line (ATCC CRL-11610) [PubMed: 9214605] and RWPE2-W99 (ATCC CRL-2853) cell line. RWPE-1 was treated with N-methyl-N-nitrosourea (MNU) to establish a series of tumorigenic cell lines that mimic the different stages of prostate cancer progression. They were WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850, WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852) cell lines. It is reported by provider that RWPE-1 cell line was tested to be negative for hepatitis B, hepatitis C, human immunodeficiency virus. Mycoplasma has been detected in this library. STR has been detected in this library. adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=1659 A类 P(52+5) RWPE-1 细胞 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 人正常前列腺上皮细胞；RWPE-1 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 肿瘤抑制基因： p53 + [PubMed: 9214605] pRB + [PubMed: 9214605] 一位正常男性前列腺组织切片的周围区域的上皮细胞用单拷贝的人乳头瘤病毒的18(HPV-18)进行转化，建立了RWPE-1 (ATCC CRL-11609) 细胞株 [PubMed: 9214605]. 在三维Matrigel培养时，在雄激素作用下，RWPE-1细胞形成腺胞并向培养基中分泌PSA。[PubMed: 11170142]. 当与Matrigel或基质细胞混合注射雄性裸鼠时，RWPE 贴壁生长 这株细胞的基本培养基作为Invitrogen (GIBCO)提供的kit的部分： 角化细胞无血清培养基K-SFM，kit目录号17005-042。随kit提供这株细胞生长所需的两种添加剂（牛垂体提取物BPE及人重组表皮生长因子EGF）。 配制完全生长培养基，需添加以下成分： 0.05 mg/ml BPE – 随K-SFM提供 5 ng/ml EGF -随K-SFM提供。 阴性