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    <!-- http://purl.obolibrary.org/obo/CLO_0004008 -->

    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0004008">
        <rdfs:label xml:lang="en">Hs 578Bst cell</rdfs:label>
        <rdfs:label xml:lang="zh">Hs 578Bst 细胞</rdfs:label>
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    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0052252">
        <rdfs:label xml:lang="en">NICR475 cell</rdfs:label>
        <rdfs:label xml:lang="zh">NICR475 细胞</rdfs:label>
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0004008"/>
        <ns2:CLO_0051622 xml:lang="en">3131C0001000200016</ns2:CLO_0051622>
        <ns2:CLO_0051629 xml:lang="en">Base Medium+8%DMSO+20%FBS</ns2:CLO_0051629>
        <ns2:CLO_0051636 xml:lang="en">Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</ns2:CLO_0051636>
        <ns2:CLO_0051633 xml:lang="en">Class A</ns2:CLO_0051633>
        <ns2:CLO_0051637 xml:lang="en">Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn.</ns2:CLO_0051637>
        <ns2:CLO_0051627 xml:lang="en">DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10%；30ng/ml EGF</ns2:CLO_0051627>
        <ns2:CLO_0051627 xml:lang="en">DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%；30ng/ml EGF</ns2:CLO_0051627>
        <ns2:CLO_0051638 xml:lang="en">Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days.</ns2:CLO_0051638>
        <ns2:IAO_0000118 xml:lang="en">Hs 578Bst</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">Hs 578Bst cell</ns2:IAO_0000118>
        <ns2:CLO_0051626 xml:lang="en">Hs 578Bst is derived from normal breast tissue adjacent to an invasive ductal carcinoma (the origin of Hs 578T). Hs 578Bst may be of myoepithelial origin because it has microfilaments and tufted vacuoles similar to those seen in the muscular epithelium of the body. No desmosomes and estrogen receptor proteins are observed, and no endophytic virus are detected.</ns2:CLO_0051626>
        <ns2:CLO_0051635 xml:lang="en">Hs578Bst-cell morphology-SHCRC</ns2:CLO_0051635>
        <ns2:CLO_0051623 xml:lang="en">Human Normal Mammary Cells; Hs 578Bst</ns2:CLO_0051623>
        <ns2:IAO_0000118 xml:lang="en">NICR475</ns2:IAO_0000118>
        <ns2:CLO_0051630 xml:lang="en">Negative</ns2:CLO_0051630>
        <ns2:CLO_0051628 xml:lang="en">P(10+5)</ns2:CLO_0051628>
        <rdfs:comment xml:lang="en">The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains).</rdfs:comment>
        <ns2:CLO_0051625 xml:lang="en">adherent growth</ns2:CLO_0051625>
        <ns2:CLO_0051624 xml:lang="en">fibroblasts</ns2:CLO_0051624>
        <rdfs:seeAlso xml:lang="en">http://www.cellresource.cn/fdetail.aspx?id=475</rdfs:seeAlso>
        <ns2:CLO_0051627 xml:lang="en">http://www.cellresource.cn/pdf/20080108100710.pdf</ns2:CLO_0051627>
        <ns2:CLO_0051633 xml:lang="zh">A类</ns2:CLO_0051633>
        <ns2:CLO_0051627 xml:lang="zh">DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%；30ng/ml EGF</ns2:CLO_0051627>
        <ns2:IAO_0000118 xml:lang="zh">Hs 578Bst 细胞</ns2:IAO_0000118>
        <ns2:CLO_0051626 xml:lang="zh">Hs 578Bst来源于一个浸润性导管癌(Hs 578T的起源)旁的正常乳房组织。 Hs 578Bst 可能是肌上皮起源的，因为它有与体内的肌上皮中见到的类似的微丝和丛生的胞饮液泡。 未观察到桥粒，没有雌激素受体蛋白，未检测到内生病毒。</ns2:CLO_0051626>
        <ns2:CLO_0051635 xml:lang="zh">Hs578Bst-细胞形态-SHCRC</ns2:CLO_0051635>
        <ns2:CLO_0051628 xml:lang="zh">P(10+5)</ns2:CLO_0051628>
        <ns2:CLO_0051627 xml:lang="zh">http://www.cellresource.cn/pdf/20080108100710.pdf</ns2:CLO_0051627>
        <ns2:CLO_0051636 xml:lang="zh">中国科学院上海生命科学研究院细胞资源中心</ns2:CLO_0051636>
        <ns2:CLO_0051623 xml:lang="zh">人正常乳腺细胞；Hs 578Bst</ns2:CLO_0051623>
        <ns2:CLO_0051629 xml:lang="zh">基础培养基+8%DMSO+20%FBS</ns2:CLO_0051629>
        <ns2:CLO_0051624 xml:lang="zh">成纤维细胞</ns2:CLO_0051624>
        <rdfs:comment xml:lang="zh">本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。</rdfs:comment>
        <ns2:CLO_0051638 xml:lang="zh">消化3-5分钟。1:2。3天内可长满。</ns2:CLO_0051638>
        <ns2:CLO_0051637 xml:lang="zh">联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn</ns2:CLO_0051637>
        <ns2:CLO_0051625 xml:lang="zh">贴壁生长</ns2:CLO_0051625>
        <ns2:CLO_0051630 xml:lang="zh">阴性</ns2:CLO_0051630>
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