SiHa cell SiHa 细胞 NICR681 cell NICR681 细胞 3131C0001000700113 Amelogenin:X；CSF1PO:12；D13S317:11；D16S539:12；D18S51:15；D19S433:14.2；D21S11:29,31；D2S1338:24；D3S1358:16,17；D5S818:9；D7S820:10；D8S1179:13,16；FGA:21；TH01:6,9；TPOX:8；vWA:14,17 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human Cervical Squamous Cell Carcinoma Cells; SiHa MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 NICR681 Negative P(6+5) SiHa SiHa cell SiHa-cell morphology-SHCRC The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cell line was constructed from an in situ tissue sample of surgical procedures from a Japanese patient. Electron microscopic observation showed that there were typical desmosomes at the junction of the cells, and there were abundant tension filaments in the cytoplasm. In nude mice, the cells were able to form poorly differentiated epidermoid carcinoma (grade III). Oncogene: PRB and P53 detection were positive. adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=681 http://www.cellresource.cn/pdf/20080108100951.pdf Amelogenin:X；CSF1PO:12；D13S317:11；D16S539:12；D18S51:15；D19S433:14.2；D21S11:29,31；D2S1338:24；D3S1358:16,17；D5S818:9；D7S820:10；D8S1179:13,16；FGA:21；TH01:6,9；TPOX:8；vWA:14,17 A类 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 P(6+5) SiHa 细胞 SiHa-细胞形态-SHCRC http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 人子宫颈鳞状细胞癌细胞；SiHa 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 该细胞系建自一个日本病人的外科手术的原位组织样品。 电镜观察表明在细胞连接处有典型的桥粒，在胞质中有丰富的张力丝。 在裸鼠中，此细胞能形成低分化的表皮样癌（三级）。 癌基因: PRB和P53阳性。 贴壁生长 阴性