104C1 cell 104C1 细胞 NICR418 cell NICR418 细胞 104C1 104C1 cell 104C1 cells was initially derived from the fetal tissue of strain 2 guinea pigs, then the cells was treated with benzopyrene for 7 days to induce malignant transformation, and the passages of cell line were obtained after continuous cloning. The cell chromosome retains diploid characteristics, but inoculation of guinea pigs produces solid tumors. 104C1-cell morphology-NIFDC 3111C0002000000046 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） Guinea Pig Embryo Cells; 104C1 NICR418 National Institute for Food and Drug Control P32 Passage at 1: 3 ~ 1: 8 RPMI 1640 (w/o Hepes) new-born calf serum，10% RPMI 1640 (w/o Hepes) 新生牛血清，10% Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=418 http://www.cellresource.cn/pdf/20080108101040.pdf 104C1 细胞 104C1-细胞形态-NIFDC 104C1细胞初期源于2/N株豚鼠的胎组织，后来细胞群体用苯并芘(Benzopyrene)处理7天诱导细胞产生恶性转化，连续克隆后获得传代细胞系。细胞染色体保留2倍体特征，但接种豚鼠产生实体肿瘤。 1：3~1：8 A类 P32 RPMI 1640 (w/o Hepes) 新生牛血清，10% http://www.cellresource.cn/pdf/20080108101040.pdf 中国食品药品检定研究院 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 成纤维细胞样 豚鼠胚胎细胞；104C1 贴壁生长