immortal mouse muscle cell line cell 永生的小鼠肌肉细胞系细胞 NICR99 cell NICR99 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000099 69~77 Base Medium+8%DMSO+20%FBS C2C12 C2C12 cell C2C12-Species Identification-PUMC C2C12-cell morphology-PUMC C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS；4mM glutamine Maintain the cell concentration between 1.5 to 10 � 105 viable cells/75 cm2; and change fluid once in 2~3 days. Mouse Myoblasts; C2C12 NICR99 This cell line is a substrain of mouse myoblast line established by Yaffe D and Saxel O. The cells differentiate rapidly and form microtubules that can contract and produce specific muscle proteins. Under the action of bone morphogenetic protein (BMP-2), the cells can differentiate from myoblasts to osteoblasts. The cell was found to be negative for ectromelia virus detection. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth fibroblast-like; spindle http://www.cellresource.cn/fdetail.aspx?id=99 http://www.cellresource.cn/pdf/20080108100710.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 69~77 A类 C2C12 细胞 C2C12-物种鉴定-PUMC C2C12-细胞形态-PUMC C5 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 20%FBS；4mM glutamine http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠成肌细胞；C2C12 成纤维细胞样；梭形 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 维持细胞浓度在1.5~10×105 viable cells/75 cm2；2~3天换液1次。 该细胞株是Yaffe D, Saxel O建立的小鼠成肌细胞系的亚株。该细胞分化较快，可形成能收缩的微管，产生特异的肌肉蛋白。在骨形态形成蛋白（BMP-2）的作用下，该细胞可由成肌细胞分化为成骨细胞。检测发现该细胞鼠痘病毒阴性。 贴壁生长