produces J774A.1 cell J774A.1 细胞 NICR415 cell NICR415 细胞 3111C0002000000043 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) new-born calf serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% J774A.1 J774A.1 cell J774A.1-cell morphology-NIFDC Monocyte/macrophage-like Mouse Mononuclear-macrophage Cells; J774A.1 NICR415 National Institute for Food and Drug Control Passage at 1: 3 ~ 1: 6 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net The cells are derived from BABL/cN mice and had antibody-dependent phagocytosis, whose growth is inhibited by dextran sulfate, PPD and LPS, and can produce IL-1β and a large amount of lysozyme. adherent growth http://www.cellresource.cn/fdetail.aspx?id=415 http://www.cellresource.cn/pdf/20080108100710.pdf 1：3~1：6 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% J774A.1 细胞 J774A.1-细胞形态-NIFDC http://www.cellresource.cn/pdf/20080108100710.pdf 中国食品药品检定研究院 单核细胞/巨噬细胞样 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 小鼠单核巨噬细胞；J774A.1 该细胞来源于BABL/cN小鼠，有抗体依赖的吞噬作用，生长受硫酸葡聚糖、PPD和LPS的抑制；可产生IL-1β和大量的溶菌酶。 贴壁生长 interleukin-1 beta