RAW 264.7 cell RAW 264.7 细胞 NICR146 cell NICR146 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000146 32~41 Base Medium+8%DMSO+20%FBS C3 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Mouse Mononuclear-macrophage Leukemia Cells; RAW 264.7 [RAW264.7 NICR146 Passage at 1: 3 ~ 1: 6; and change fluid once in 2~3 days. RAW 264.7 RAW 264.7 cell RAW264.7 RAW264.7 cell RAW264.7-Species Identification-PUMC This cell line is derived from a tumor induced by Abelson murine leukemia virus in BALB/c mice. The cells produce Lysozyme can be produced, and detections for sIg-, Ia-antigen and Thy-1.2 surface antigen are negative. To detect the secretion of viral particles, the XC spot formation assay is negative. The cells could drink neutral red and devour latex particles and zymosan, and could divide sheep red blood cells and tumor target cells in an antibody-dependent manner; LPS or PPD treatment for 2 days can induce split of red blood cells but had no effect on tumor target cells. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth http://www.cellresource.cn/fdetail.aspx?id=146 http://www.cellresource.cn/pdf/20080108100710.pdf irregular round - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:6传代；2~3天换液1次。 32~41 A类 C3 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS RAW 264.7 细胞 RAW264.7 细胞 RAW264.7-物种鉴定-PUMC http://www.cellresource.cn/pdf/20080108100710.pdf 不规则圆形 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠单核巨噬细胞白血病细胞；RAW 264.7 [RAW264.7 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 此细胞株源自BALB/c小鼠由Abelson鼠科白血病病毒诱导的肿瘤。可产生溶菌酶；sIg-, Ia-，Thy-1.2-。为检测到病毒颗粒的分泌，XC斑点形成试验阴性。该细胞可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖；可以经抗体依赖分裂绵羊红细胞与肿瘤靶细胞；LPS或PPD处理2天可诱导分裂红细胞，但对肿瘤靶细胞无作用。 贴壁生长