F9 cell F9 细胞 NICR731 cell NICR731 细胞 3131C0001000800004 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. F9 F9 cell F9 cells differentiate into somatic endoderm under the stimulation of tretinoin and cyclic adenosine monophosphate (cAMP). The differentiated cells synthesize plasminogen activator, laminin and Collagen Type Ⅳ. CAMP only works on cells treated with tretinoin. There are 3 copies of β1 integrin gene in the cells. There are 3 copies of β1 integrin gene in the cells. Tests showed that the cells were ectromelia virus-negative. Mouse Testicular Teratoma Cells; F9 NICR731 Negative PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=731 http://www.cellresource.cn/pdf/20080108100710.pdf A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% F9 细胞 PN5 http://www.cellresource.cn/pdf/20080108100710.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 在维生素A酸和联丁酰基cAMP刺激下，F9细胞分化成体壁内皮层。 分化的细胞合成血浆酶原活化因子，粘层蛋白和IV 型胶原质。 只有在经过维生素A酸处理后细胞上的cAMP才是活化的。 细胞中有三个拷贝β1粘合素基因。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 基础培养基+8%DMSO+20%FBS 小鼠睾丸畸胎瘤细胞；F9 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性