P19 cell P19 细胞 NICR20 cell NICR20 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000020 38~45 Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Do not make the cells grow to confluence, and passage at 1: 10; and passage once in 2 to 3 days. Mouse Testicular Teratoma Cells; P19 NICR20 P19 P19 cell P19 cell line was established from malignant teratomas induced in C3H/He mice. The cells can be efficiently cloned in a medium containing 0.1 mM 2-mercaptoethanol. The cells are multipotent and can differentiate into nerve and glial-like cells under the induction of 500 nM retinoic acid; in the presence of 0.5% -1.0% dimethyl sulfoxide (DMSO), they differentiate into neuroglial-like cells, but does not form nerve or glial-like cells; In the presence of DMSO and retinoic acid, the differentiation of cells is the same as that of the cells cultured in retinoic acid alone. P19-Cell Morphology-PUMC P19-Species Identification-PUMC P19-cell morphology-PUMC Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=20 http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 10%FBS - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 38~45 A类 C5 P19 细胞 P19-物种鉴定-PUMC P19-细胞形态-PUMC P19细胞株是从C3H/He小鼠中诱导的恶性畸胎瘤中建立的。该细胞在含有0.1mM 2-巯基乙醇的培养基中可高效率地克隆。该细胞具有多能性，在500nM 维A酸诱导下可以分化成神经和神经胶质样细胞；在0.5%~1.0%二甲亚砜(DMSO) 存在下，分化形成心脏和骨骼肌样细胞，但不形成神经或神经胶质样细胞；在DMSO和维A酸同时存在时，细胞的分化与只有维A酸一样。 http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 10%FBS 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 勿使细胞长至汇合状态，1:10传代；2~3天1次。 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠睾丸畸胎瘤细胞；P19 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长