P19 cell P19 细胞 NICR750 cell NICR750 细胞 3131C0001000800027 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Mouse Testicular Teratoma Cells; P19 NICR750 Negative P19 P19 cell P19 cell lines were established from teratoma induced in C3H/He mice. The efficiency of cloning in medium containing 0.1 mM 2-mercaptoethanol is high. The cells are multipotent. Cells can differentiate into nerve and glial-like cells under the induction of 500 nM vitamin A acid. In that presence of 0.5% to 1.0% dimethyl sulfoxide (DMSO), the cell differentiate into cardiac and skeletal muscle-like matrix, but does not form nerve or glial-like cells. In the presence of DMSO and vitamin A acid, the differentiation of the cells is the same as that of the cells cultured in retinoic acid alone. PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=750 http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) ?calf serum，7.5% fetal bovine serum，2.5% α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 小牛血清，7.5%；胎牛血清，2.5% A类 P19 细胞 P19细胞株是从C3H/He小鼠中诱导的畸胎癌中建立的。 在含有0.1mM 2-巯基乙醇的培养基中克隆的效率高。 细胞具有多能性。 在500nM 维生素A酸诱导下细胞可以分化成神经和神经胶质样细胞。 在0.5%到1.0% 二甲亚砜(DMSO) 存在下，细胞分化形成心脏和骨骼肌样基质，但不形成神经或神经胶质样细胞。 在DMSO和维生素A酸同时存在时，细胞的分化与只有维生素A酸一样。 PN5 http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 小牛血清，7.5%；胎牛血清，2.5% 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠睾丸畸胎瘤细胞；P19 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性