TM3 cell TM3 细胞 NICR1667 cell NICR1667 细胞 3131C0001000300024 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) horse serum，5% qualified fetal bovine serum，2.5% DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 马血清，5%；优质胎牛血清，2.5% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Mouse Leydig Cells; TM3 NICR1667 Negative PN5 TM3 TM3 cell TM3 cells increased the cAMP production under the action of LH, but did not respond to follicle stimulating hormone. The response duration to LH was related to serum batches. In the presence of LH, the cell can metabolize cholesterol. The cells were found to be ectromelia virus-negative. TM3-cell morphology-SHCRC The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=1667 http://www.cellresource.cn/pdf/20080108100542.pdf A类 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 马血清，5%；优质胎牛血清，2.5% PN5 TM3 细胞 TM3-细胞形态-SHCRC TM3细胞在LH作用下cAMP产量升高，但对促卵泡激素没有响应。对LH的响应持续时间与血清批次有关。在LH存在下，细胞可以代谢胆固醇。检测发现鼠痘病毒阴性。 http://www.cellresource.cn/pdf/20080108100542.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠睾丸间质细胞；TM3 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性