P3/NSI/1-Ag4-1 (NS-1) cell P3/NSI/1-Ag4-1 (NS-1) 细胞 NICR413 cell NICR413 细胞 3111C0002000000041 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) new-born calf serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% Mouse Myeloma Cells; P3/NSI/1-Ag4-1 [NS-1] NICR413 NS-1 NS-1 cell National Institute for Food and Drug Control P3/NSI/1-Ag4-1 P3/NSI/1-Ag4-1 cell Passage at 1: 4 ~ 1: 8 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net This cell is a non-secreted clone of P3X63Ag8 (ATCC TIB-9). Kappa chains are synthesized but not secreted. The cells are resistant to 0.1 mM 8-azaguanine but unable to grow in HAT medium. It is reported that it is cholesterol-deficient to the lack of 3-ketosteroid reductase activity. Detection for limb bone malformations virus ectromelia virus is negative. http://www.cellresource.cn/fdetail.aspx?id=413 http://www.cellresource.cn/pdf/20080108100710.pdf lymphoblast-like suspension growth 1：4~1：8 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% NS-1 细胞 P3/NSI/1-Ag4-1 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf 中国食品药品检定研究院 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 小鼠骨髓瘤细胞；P3/NSI/1-Ag4-1 [NS-1] 悬浮生长 淋巴母细胞样 该细胞是P3X63Ag8 (ATCC TIB-9)的一个不分泌克隆。 Kappa链合成了但不分泌。 能抗0.1 mM 8-氮杂鸟嘌呤但不能在HAT培养基中生长。 据报道它是由于缺失了3-酮类固醇还原酶活性的胆固醇营养缺陷型。检测表明肢骨发育畸形病毒(鼠痘)阴性。