P3/NSI/1-Ag4-1 (NS-1) cell P3/NSI/1-Ag4-1 (NS-1) 细胞 NICR748 cell NICR748 细胞 3131C0001000800025 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% Mouse Myeloma Cells; P3/NSI/1-Ag4-1 [NS-1] NICR748 NS-1 NS-1 cell NS-1-cell morphology-SHCRC Negative P3/NSI/1-Ag4-1 P3/NSI/1-Ag4-1 cell PN5 Passage at 1: 2. The cells can be overgrown within 3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell is a non-secreted clone of P3X63Ag8 (ATCC TIB-9). Kappa chains are synthesized but not secreted. The cells are resistant to 0.1 mM 8-azaguanine but unable to grow in HAT medium. It is reported that it is cholesterol-deficient to the lack of 3-ketosteroid reductase activity. Detection showed that the cells is limb bone malformations virus (ectromelia virus)-negative. http://www.cellresource.cn/fdetail.aspx?id=748 http://www.cellresource.cn/pdf/20080108100710.pdf lymphoblast suspension growth 1:2。3天内可长满。 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% NS-1 细胞 NS-1-细胞形态-SHCRC P3/NSI/1-Ag4-1 细胞 PN5 http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠骨髓瘤细胞；P3/NSI/1-Ag4-1 [NS-1] 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 淋巴母细胞 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 这是P3X63Ag8 (ATCC TIB-9)的一个不分泌克隆。 Kappa链合成了但不分泌。 能抗0.1 mM 8-氮杂鸟嘌呤但不能在HAT培养基中生长。 据报道它是由于缺失了3-酮类固醇还原酶活性的胆固醇营养缺陷型。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 阴性