immortal mouse cell line cell 永生的小鼠细胞系细胞 NICR782 cell NICR782 细胞 3142C0001000000014 Base Medium+8%DMSO+20%FBS C4 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn Isoenzyme Identification Chromosome Mouse Myeloma Cells; P3X63-Ag8.653 NICR782 Negative P3X63-Ag8.653 P3X63-Ag8.653 cell Passage at 1: 3, and passage once in 2-3 days. RPMI 1640 (w/o Hepes) 10%FBS The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell line is resistant to 8-azaguanine but sensitive to HAT. It can be used as a fusion ligand for hybridomas. It is capable of producing but not secreting immunoglobulin. It is reported that it is cholesterol-deficient to the lack of 3-ketosteroid reductase activity. Detection for limb bone malformations virus ectromelia virus is negative. http://www.cellresource.cn/fdetail.aspx?id=782 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like suspension growth 1:3传代,2-3天传一代 A类 C4 P3X63-Ag8.653 细胞 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 中国典型培养物保藏中心细胞库 同工酶鉴定 基础培养基+8%DMSO+20%FBS 小鼠骨髓瘤细胞；P3X63-Ag8.653 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 淋巴母细胞样 这株细胞能抗8-氮杂鸟嘌呤但对HAT敏感。 可以用作杂交瘤时的融合配体。 能生成但不分泌免疫球蛋白。 据报道它是由于缺失了3-酮类固醇还原酶活性的胆固醇营养缺陷型。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性