SP2/0-Ag14 cell SP2/0-Ag14 细胞 NICR383 cell NICR383 细胞 3111C0002000000011 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) new-born calf serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% Mouse Myeloma Cells; Sp2/0-Ag14 NICR383 National Institute for Food and Drug Control Passage at 1: 4-1: 8 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net Sp2/0-Ag14 Sp2/0-Ag14 cell The cells were obtained by fusion of spleen cells of BALB/c mice immunized with sheep erythrocytes and P3X63Ag8 myeloma cells. This cell, which does not secrete immunoglobulin, is resistant to 20 μg/ml of 8-azaguanine and sensitive to HAT, ans can be used as B cell component in cell fusion to prepare hybridoma; This cell is mouse pox virus-negative. http://www.cellresource.cn/fdetail.aspx?id=383 http://www.cellresource.cn/pdf/20080108100710.pdf lymphoblast-like; round suspension growth 1：4-1：8 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 新生牛血清，10% Sp2/0-Ag14 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf 中国食品药品检定研究院 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 小鼠骨髓瘤细胞；Sp2/0-Ag14 悬浮生长 淋巴母细胞样；圆形 该细胞是由绵羊红细胞免疫的BALB/c小鼠脾细胞和P3X63Ag8骨髓瘤细胞融合得到的。该细胞不分泌免疫球蛋白，对20 μg/ml的8-氮鸟嘌呤有抗性，对HAT比较敏感；该细胞可以作为细胞融合时的B细胞组分用于制备杂交瘤；鼠痘病毒阴性。