CT26.WT cell CT26.WT 细胞 NICR275 cell NICR275 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000275 Base Medium+8%DMSO+20%FBS C2 CT26 is an undifferentiated colon cancer cell line induced by N-nitroso-N-methylurethane-(NNMU). One of its cloned cell lines was named as CT26.WT. CT26. WT is stably transformed by retroviral vector LXSN and form a lethal subclone CT26. CL25, which contains the lacZ gene, encoding tumor associated antigen (TAA) and beta galactosidase. CT26. WT and CT26. CL25 cells are similar in growth rate and lethality in mice, except that CT26. CL25 cells can express tumor-associated antigen and beta galactosidase, thus these two cells can be used for the research of Immunotherapy and host immune response. CT26.WT CT26.WT cell CT26.WT-Species Identification-PUMC CT26WT CT26WT cell Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Mouse Colon Cancer Cells; CT26.WT [CT26WT] NICR275 Passage at 1: 4 ~ 1: 10, and change fluid once in 2~3 days. RPMI 1640 (w/o Hepes) 10%FBS Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=275 http://www.cellresource.cn/pdf/20080108101040.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:4~1:10传代，2~3天换液1次。 A类 C2 CT26.WT 细胞 CT26.WT-物种鉴定-PUMC CT26WT 细胞 CT26细胞是被N-亚硝基-N-甲基脲烷（NNMU）诱导得到的未分化的小鼠结肠癌细胞，该细胞的一个克隆形成的细胞系被命名为CT26.WT。CT26.WT被逆转录病毒载体LXSN稳定转化形成了一个致死性的亚克隆CT26.CL25，这一病毒载体含有lacZ基因、编码肿瘤相关抗原（TAA）和beta半乳糖苷酶。CT26.WT和CT26.CL25细胞在小鼠中生长速度和致死率都很相似，不同的是CT26.CL25细胞可以表达肿瘤相关抗原和beta半乳糖苷酶，因此这两株细胞可以联合用于免疫治疗和宿主免疫反应的研究。 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠结肠癌细胞；CT26.WT [CT26WT] 成纤维细胞样 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长